Glycosphingolipids of porcine, bovine, and equine pericardia as potential immune targets in bioprosthetic heart valve grafts
| Autor: | Chunsheng Jin, Susann Teneberg, John Benktander, Christy Whiddon, Angela Barone, Cesare Galli, Michael E. Breimer |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.drug_class Swine Immunology Transplantation Heterologous Monoclonal antibody Glycosphingolipids 03 medical and health sciences chemistry.chemical_compound Glycolipid Immune system Antigen medicine Pericardium Animals Humans Horses Bioprosthesis Transplantation 030102 biochemistry & molecular biology biology Antibodies Monoclonal Glycosphingolipid Carbohydrate biology.organism_classification Heart Valves 030104 developmental biology medicine.anatomical_structure Biochemistry chemistry Heart Valve Prosthesis Cattle Neuraminic Acids Bacteria |
| Zdroj: | Xenotransplantation. 25(5) |
| ISSN: | 1399-3089 |
| Popis: | Background Pericardial tissue from various animal species is utilized for the production of the bioprosthetic heart valves (BHV) used clinically. Experimental data show that the eventual breakdown of BHV is partly due to immunological interactions with carbohydrate tissue antigens. To understand these processes, we have examined the glycolipid-based carbohydrate antigens in naive porcine, bovine, and equine pericardia. Experimental Total non-acid and acid glycosphingolipid fractions were isolated from porcine, bovine, and equine pericardia, and individual glycolipid compounds were characterized by thin-layer chromatography, mass spectrometry, and binding of monoclonal antibodies, lectins and bacteria in chromatogram binding assays. Results The non-acid glycolipid fractions from all species contained glycosphingolipids based on the globo- and neolacto-series, including pentaglycosylceramides with terminal Galα3 determinants. Terminal blood group A and H (O) structures based on type 2 core chains were present in porcine pericardium, while the Forssman pentaosylceramide was found in equine pericardium. All acid glycolipid fractions contained sulfatide and several gangliosides with both N-acetyl- and N-glycolyl-neuraminic acid as terminal saccharide chain determinants. Conclusion Several carbohydrate antigens which are potential targets for the human immune system have been identified in the animal pericardial tissues used for the production of BHV. Which of these antigens are left in the tissues after industrial BHV production processes, as well as their potential role in eventual BHV degradation, remains to be elucidated. |
| Databáze: | OpenAIRE |
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