Production of IL-1β, IL-6 and CXCL8 by endometrium of crossbred heifers stimulated with various pathogen-associated molecular patterns
| Autor: | Telma da Mata Martins, Philipe Pimenta Nunes, Tatiane A. Paixão, Eliane Beatriz Magalhães Silva, Ana Carolina Leite, Renato L. Santos, Álan Maia Borges |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
Chemokine Lipopolysaccharide animal diseases medicine.medical_treatment Interleukin-1beta Estrous Cycle Peptidoglycan Biology Endometrium Andrology Tissue Culture Techniques 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Food Animals medicine Animals Interleukin 8 Estrous cycle 030219 obstetrics & reproductive medicine Interleukin-6 Interleukin-8 0402 animal and dairy science Interleukin 04 agricultural and veterinary sciences General Medicine Zebu 040201 dairy & animal science Cytokine medicine.anatomical_structure chemistry Gene Expression Regulation biology.protein Animal Science and Zoology Cattle Female |
| Zdroj: | Animal reproduction science. 228 |
| ISSN: | 1873-2232 |
| Popis: | Uterine bacterial infections are common during the post-partum period of dairy herds and, apparently, incidences in crossbred cattle are less than in Holsteins. The aims of this study were (I) to evaluate production of interleukin 1-β (IL-1β), interleukin-6 (IL-6) and chemokine CXCL8 using endometrial explants from Bos indicus crossbred heifers at diestrous, stimulated by various pathogen-associated molecular patterns (PAMP), and (II) assess production of these cytokines by lipopolysaccharide-stimulated endometrial explants from heifers when samples were collected at different stages of estrous cycle. In the first experiment, endometrial explants from heifers at diestrous were stimulated by ten-fold serial dilutions of lipopolysaccharide (LPS), triacylated lipopeptide (PAM3) or peptidoglycan (PGN). In the second experiment, endometrial explants collected at different stages of the estrous cycle were treated with LPS. Concentrations of IL-1β, IL-6 and CXCL8 were quantified in supernatant. There was a marked (P 0.05) production of IL-1β, IL-6, and CXCL8 in response to LPS treatment. There was also production of IL-1β (P 0.05) in response to PGN treatment. Explant samples collected at different stages of the estrous cycle responded to LPS treatment with production of IL-1β and IL-6, but with no differences (P 0.05) between stages of estrous cycle. In conclusion, endometrial samples of crossbred Zebu-based heifers collected during diestrous produced IL-1β, IL-6 and CXCL8 in response to LPS and IL-1β in response to PGN. The cytokine production in response to LPS, however, was not affected by the stage of the estrous cycle in Bos indicus crossbred heifers. |
| Databáze: | OpenAIRE |
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