Role of cardiac TBX20 in dilated cardiomyopathy
Autor: | Anupam Mittal, Madhu Khullar, Ajay Bahl, Rishikesh Prasad, Rajni Sharma |
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Rok vydání: | 2016 |
Předmět: |
Adult
Cardiomyopathy Dilated Male 0301 basic medicine medicine.medical_specialty DNA Copy Number Variations TBX20 Clinical Biochemistry Cardiomyopathy 030204 cardiovascular system & hematology Sudden cardiac death 03 medical and health sciences 0302 clinical medicine Internal medicine Animals Humans Medicine cardiovascular diseases Promoter Regions Genetic Molecular Biology Ejection fraction business.industry Dilated cardiomyopathy Cell Biology General Medicine DNA Methylation Middle Aged medicine.disease Pathophysiology Rats Disease Models Animal 030104 developmental biology medicine.anatomical_structure Ventricle Heart failure cardiovascular system Cardiology Female T-Box Domain Proteins business |
Zdroj: | Molecular and Cellular Biochemistry. 414:129-136 |
ISSN: | 1573-4919 0300-8177 |
Popis: | Dilated cardiomyopathy (DCM) is an important cause of heart failure and sudden cardiac death worldwide. Transcription factor TBX20 has been shown to play a crucial role in cardiac development and maintenance of adult mouse heart. Recent studies suggest that TBX20 may have a role in pathophysiology of DCM. In the present study, we examined TBX20 expression in idiopathic DCM patients and in an animal model of cardiomyopathy, and studied its correlation with echocardiographic indices of LV function. Endomyocardial biopsies (EMBs) from intraventricular septal from the right ventricle region were obtained from idiopathic DCM patients (IDCM, n = 30) and from patients with ventricular septal defect (VSD, n = 14) with normal LVEF who served as controls. An animal model of DCM was developed by right renal artery ligation in Wistar rats. Cardiac TBX20 mRNA levels were measured by real-time PCR in IDCM, controls, and in rats. The role of DNA promoter methylation and copy number variation (CNVs) in regulating TBX20 gene expression was also investigated. Cardiac TBX20 mRNA levels were significantly increased (8.9 fold, p < 0.001) in IDCM patients and in RAL rats as compared to the control group. Cardiac TBX20 expression showed a negative correlation with LVEF (r = -0.71, p < 0.001) and a positive correlation with left ventricular end-systolic volume (r = 0.39, p = 0.038). No significant difference in TBX20 CNVs and promoter methylation was observed between IDCM patients and control group. Our results suggest a potential role of TBX20 in pathophysiology of DCM. |
Databáze: | OpenAIRE |
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