Sijunzi Decoction Inhibits Stemness by Suppressing β-Catenin Transcriptional Activity in Gastric Cancer Cells
| Autor: | Ping Tang, Hong Wang, Yue-Jun Li, Qing-Hua Peng, Lin-Li Liao, Pei Liu |
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| Rok vydání: | 2021 |
| Předmět: |
biology
Cell growth Chemistry CD44 DNA General Medicine Molecular biology Complementary and alternative medicine Stomach Neoplasms Cell culture Cancer stem cell Apoptosis Cell Line Tumor Cancer cell Neoplastic Stem Cells biology.protein Humans Pharmacology (medical) Viability assay Stem cell beta Catenin Drugs Chinese Herbal |
| Zdroj: | Chinese Journal of Integrative Medicine. 28:702-710 |
| ISSN: | 1993-0402 1672-0415 |
| DOI: | 10.1007/s11655-021-3314-9 |
| Popis: | OBJECTIVE To investigate a previously uncharacterized function of Sijunzi Decoction (SJZD) in inhibition of gastric cancer stem cells (GCSCs). METHODS MKN74 and MKN45, two CD44 positive gastric cancer cell lines with stem cell properties were used. The cells were divided into 2 groups. Treatment group was treated with SJZD (1-5 mg/mL) for indicated time (48 h-14 days). The control group was treated with equal volume of phosphate buffered saline. Cell Counting Assay Kit-8 were used to measure cell viability. Spheroid colony formation and GCSCs marker expression were performed to determine GCSCs stemness. Cell fractionation and chromatin immunoprecipitation assays were used to assess the distribution and DNA-binding activity of β-catenin after SJZD treatment, respectively. RESULTS SJZD treatment repressed cell growth and induced apoptosis in MKN74 and MKN45 cell lines (P |
| Databáze: | OpenAIRE |
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