Assessment on Experimental Bacterial Biofilms and in Clinical Practice of the Efficacy of Sampling Solutions for Microbiological Testing of Endoscopes
| Autor: | Claire Hennequin, E. Thibert, Ousmane Traore, F. A. Chapelle, Olivier Lesens, Claire Aumeran |
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| Přispěvatelé: | Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Centre National de la Recherche Scientifique (CNRS), Service d'Hygiène Hospitalière [CHU Clermont-Ferrand], CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand |
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Endoscope MESH: Random Allocation medicine.medical_treatment MESH: Biofilms MESH: Bacteriological Techniques Biology MESH: Bacterial Load Specimen Handling Microbiology Random Allocation 03 medical and health sciences 0302 clinical medicine medicine Humans Prospective Studies Food science MESH: Specimen Handling MESH: Endoscopes Saline Endoscopes Bacteriological Techniques 0303 health sciences MESH: Humans Bacteria 030306 microbiology Sterile water Significant difference Biofilm Sampling (statistics) Bacteriology Contamination MESH: Hospitals [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Bacterial Load Hospitals MESH: Prospective Studies Clinical Practice MESH: Bacteria Biofilms 030211 gastroenterology & hepatology |
| Zdroj: | Journal of Clinical Microbiology Journal of Clinical Microbiology, American Society for Microbiology, 2012, 50 (3), pp.938-42. ⟨10.1128/JCM.06221-11⟩ Journal of Clinical Microbiology, 2012, 50 (3), pp.938-42. ⟨10.1128/JCM.06221-11⟩ |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Opinions differ on the value of microbiological testing of endoscopes, which varies according to the technique used. We compared the efficacy on bacterial biofilms of sampling solutions used for the surveillance of the contamination of endoscope channels. To compare efficacy, we used an experimental model of a 48-h Pseudomonas biofilm grown on endoscope internal tubing. Sampling of this experimental biofilm was performed with a Tween 80-lecithin-based solution, saline, and sterile water. We also performed a randomized prospective study during routine clinical practice in our hospital sampling randomly with two different solutions the endoscopes after reprocessing. Biofilm recovery expressed as a logarithmic ratio of bacteria recovered on bacteria initially present in biofilm was significantly more effective with the Tween 80-lecithin-based solution than with saline solution ( P = 0.002) and sterile water ( P = 0.002). There was no significant difference between saline and sterile water. In the randomized clinical study, the rates of endoscopes that were contaminated with the Tween 80-lecithin-based sampling solution and the saline were 8/25 and 1/25, respectively ( P = 0.02), and the mean numbers of bacteria recovered were 281 and 19 CFU/100 ml ( P = 0.001), respectively. In conclusion, the efficiency and therefore the value of the monitoring of endoscope reprocessing by microbiological cultures is dependent on the sampling solutions used. A sampling solution with a tensioactive action is more efficient than saline in detecting biofilm contamination of endoscopes. |
| Databáze: | OpenAIRE |
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