Chemo‐enzymatic epoxidation of linoleic acid: Parameters influencing the reaction

Autor: Samuel Camocho, Cecilia Orellana-Coca, Bo Mattiasson, Dietlind Adlercreutz, Rajni Hatti-Kaul
Rok vydání: 2005
Předmět:
Zdroj: European Journal of Lipid Science and Technology. 107:864-870
ISSN: 1438-9312
1438-7697
DOI: 10.1002/ejlt.200500253
Popis: The effect of reaction parameters on lipase-mediated chemo-enzymatic epoxidation of linoleic acid was investigated. Hydrogen peroxide was found to have the most significant effect on the reaction rate and degree of epoxidation. Excess of hydrogen peroxide with respect to the amount of double bonds was necessary in order to yield total conversion within a short time period, as well as at temperatures above 50 °C to compensate for hydrogen peroxide decomposition. However, prolonged incubation with high excess of hydrogen peroxide leads to the accumulation of peracids in the final product. The reaction rate increased also with increasing hydrogen peroxide concentration (between 10 and 50 wt-%); however, at the expense of enzyme inactivation. Linoleic acid was completely epoxidized when used at a concentration of 0.5-2 M in toluene at 30 °C, while in a solvent-free medium, the reaction was not complete due to the formation of a solid or a highly viscous oily phase, creating mass transfer limitations. Increasing the temperature up to 60 °C also improved the rate of epoxide formation.
Databáze: OpenAIRE
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