Type-specific human papillomavirus oncogene messenger RNA levels correlate with the severity of cervical neoplasia
| Autor: | Bor Heng Lee, Chih Ming Ho, Tsai Yen Chien, Chiu Cho Yan, Shwu Fen Chang, Shih Hung Huang, Wen-Fang Cheng |
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| Rok vydání: | 2010 |
| Předmět: |
Cancer Research
Uterine Cervical Neoplasms Alphapapillomavirus Biology Cervical intraepithelial neoplasia Severity of Illness Index Virus medicine Humans RNA Messenger DNA Primers Messenger RNA Base Sequence Reverse Transcriptase Polymerase Chain Reaction Nucleic Acid Hybridization virus diseases RNA Cancer Oncogenes Viral Load medicine.disease Virology Molecular biology female genital diseases and pregnancy complications Reverse transcriptase Cross-Sectional Studies Oncology Dysplasia Female Viral load |
| Zdroj: | International Journal of Cancer. 127:622-632 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.25078 |
| Popis: | This study aimed to evaluate whether quantitation of high-risk human papillomavirus (HR-HPV) E6 messenger RNA (mRNA) can be a potential biomarker for detecting the severity of cervical lesions. HPV genotyping was performed using a modified MY11/GP6+ PCR for HPV DNA amplification, followed by HPV genotype-specific hybridization with on a gene chip. E6 type-specific PCR was used to validate multiple infections. Quantitative real-time reverse transcriptase (QRT-PCR) and real-time PCR used to measure mRNA levels and DNA viral loads of 6 HPV oncogenic types (HPV 16, 18, 31, 33, 52 and 58) in 720 liquid-based cytology samples. The HPV DNA and RNA measurements were correlated with cervical lesions diagnosed by histopathologic examination. mRNA transcripts in the 6 types HPV DNA-positive cases was lower in normal women and |
| Databáze: | OpenAIRE |
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