Both Th1 and Th17 Are Immunopathogenic but Differ in Other Key Biological Activities
| Autor: | Guangpu Shi, Barbara P. Vistica, Chi-Chao Chan, Eric F. Wawrousek, Hongen Yin, Igal Gery, Catherine A. Cox |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adoptive cell transfer
Receptors CXCR3 Eye Diseases Lymphocyte Immunology Mice Transgenic chemical and pharmacologic phenomena Inflammation CD38 Biology CXCR3 CD49d Article Cell Line Interferon-gamma Mice T-Lymphocyte Subsets medicine Animals Immunology and Allergy Cells Cultured Membrane Glycoproteins Interleukin-17 Cell Polarity hemic and immune systems T-Lymphocytes Helper-Inducer Th1 Cells ADP-ribosyl Cyclase 1 Adoptive Transfer Mice Mutant Strains medicine.anatomical_structure Cell culture Cytokines medicine.symptom Homing (hematopoietic) |
| Zdroj: | The Journal of Immunology. 180:7414-7422 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | The role of Th17 lymphocytes in immunopathogenic processes has been well established, but little is known about their basic cell features. In this study, we compared polarized Th1 and Th17 for key biological activities related to pathogenicity and trafficking. Th1 and Th17 lineages were derived from TCR-transgenic CD4 murine cells specific against hen egg lysozyme. When adoptively transferred into mice expressing hen egg lysozyme in their eyes, both Th1 and Th17 induced ocular inflammation but with slight differences in histological pathology. PCR analysis revealed selective expression of IFN-γ or IL-17 in eyes of Th1 or Th17 recipients, respectively. Additionally, Th1 and Th17 were found to differ in three other key activities: 1) Th17 cells were inferior to Th1 cells in their capacity to trigger massive lymphoid expansion and splenomegaly; 2) the proportion of Th1 cells among infiltrating cells in inflamed recipient eyes declined rapidly, becoming a minority by day 7, whereas Th17 cells remained in the majority throughout this period; and 3) remarkable differences were noted between Th1 and Th17 cells in their expression of certain surface markers. In particular, reactivated Th1 expressed higher levels of CD49d and α4β7 (mucosal homing) in vitro and higher levels of CXCR3 (Th1 trafficking) in vivo. Reactivated Th17, however, expressed higher levels of αEβ7 (epithelial tissue homing) and CD38 (activation, maturation and trafficking) in vitro, but in vivo Th17 expressed higher levels of α4β7 and CCR6 (lymphocyte trafficking). These data reveal that Th1 and Th17 cells differ in several key biological activities influencing migration and pathogenic behavior during inflammatory disease. |
| Databáze: | OpenAIRE |
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