Angiotensin II receptor type 1 is upregulated in atrial tissue of patients with rheumatic valvular disease with atrial fibrillation

Autor: Mei-Lin Xu, Hongliang Cong, Yong-min Mao, Ximing Li, Hua Jiang, Li-Jun Ma
Rok vydání: 2010
Předmět:
Adult
Male
Pulmonary and Respiratory Medicine
medicine.medical_specialty
Angiotensin receptor
Biopsy
Blotting
Western

Heart Valve Diseases
Receptor
Angiotensin
Type 2

Receptor
Angiotensin
Type 1

Internal medicine
Atrial Fibrillation
Renin–angiotensin system
Humans
Medicine
Sinus rhythm
Heart Atria
RNA
Messenger

cardiovascular diseases
education
Heart Valve Prosthesis Implantation
education.field_of_study
Angiotensin II receptor type 1
biology
Reverse Transcriptase Polymerase Chain Reaction
business.industry
Rheumatic Heart Disease
Angiotensin-converting enzyme
Atrial fibrillation
Middle Aged
medicine.disease
Immunohistochemistry
Angiotensin II receptor type 2
Up-Regulation
Cardiac surgery
cardiovascular system
biology.protein
Cardiology
Female
Surgery
Cardiology and Cardiovascular Medicine
business
Zdroj: The Journal of Thoracic and Cardiovascular Surgery. 140:298-304
ISSN: 0022-5223
Popis: ObjectiveThe purpose of this study was to examine the changes in expression of angiotensin II receptor type 1/2 in left or right atrial tissue from patients with rheumatic valvular disease with or without atrial fibrillation.MethodsAtrial tissue samples were obtained from 39 patients with rheumatic mitral valve disease during cardiac surgery. Among these patients, there were 25 with atrial fibrillation and 14 with sinus rhythm. The level of angiotensin II receptor type 1 or type 2 mRNA transcription was measured by means of a semiquantitative reverse transcription–polymerase chain reaction technique. Expression of angiotensin II receptor type 1 or type 2 protein was detected by means of immunohistochemistry assay and Western blot analysis.ResultsThe inner diameter of the left atrium was clearly enlarged in the atrial fibrillation group in comparison with that seen in the sinus rhythm group. The expression levels of both angiotensin II receptor type 1 mRNA and protein in the left atrial tissue were significantly increased in the patients with atrial fibrillation compared with those seen in patients with sinus rhythm (P < .05). Interestingly, the comparison of angiotensin II receptor type 2 expression levels in the left atrial tissue between these 2 groups is not statistically significant. In addition, the results of angiotensin II receptor type 1 or 2 expression in the right atrial tissue did not show any obvious change in the patients with atrial fibrillation versus those with sinus rhythm.ConclusionsExpression of angiotensin II receptor type 1 but not type 2 is highly upregulated only in the left atrial tissue of patients with rheumatic valvular disease with atrial fibrillation. This suggests that there is a possible pathophysiologic role of the renin–angiotensin system in patients with atrial fibrillation and that a series of effects mediated by the activation of angiotensin II receptor type 1 in the left atrial tissue might be one of the molecular mechanisms involved in the process of atrial remodeling in atrial fibrillation.
Databáze: OpenAIRE