Angiotensin II receptor type 1 is upregulated in atrial tissue of patients with rheumatic valvular disease with atrial fibrillation
| Autor: | Mei-Lin Xu, Hongliang Cong, Yong-min Mao, Ximing Li, Hua Jiang, Li-Jun Ma |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine medicine.medical_specialty Angiotensin receptor Biopsy Blotting Western Heart Valve Diseases Receptor Angiotensin Type 2 Receptor Angiotensin Type 1 Internal medicine Atrial Fibrillation Renin–angiotensin system Humans Medicine Sinus rhythm Heart Atria RNA Messenger cardiovascular diseases education Heart Valve Prosthesis Implantation education.field_of_study Angiotensin II receptor type 1 biology Reverse Transcriptase Polymerase Chain Reaction business.industry Rheumatic Heart Disease Angiotensin-converting enzyme Atrial fibrillation Middle Aged medicine.disease Immunohistochemistry Angiotensin II receptor type 2 Up-Regulation Cardiac surgery cardiovascular system biology.protein Cardiology Female Surgery Cardiology and Cardiovascular Medicine business |
| Zdroj: | The Journal of Thoracic and Cardiovascular Surgery. 140:298-304 |
| ISSN: | 0022-5223 |
| Popis: | ObjectiveThe purpose of this study was to examine the changes in expression of angiotensin II receptor type 1/2 in left or right atrial tissue from patients with rheumatic valvular disease with or without atrial fibrillation.MethodsAtrial tissue samples were obtained from 39 patients with rheumatic mitral valve disease during cardiac surgery. Among these patients, there were 25 with atrial fibrillation and 14 with sinus rhythm. The level of angiotensin II receptor type 1 or type 2 mRNA transcription was measured by means of a semiquantitative reverse transcription–polymerase chain reaction technique. Expression of angiotensin II receptor type 1 or type 2 protein was detected by means of immunohistochemistry assay and Western blot analysis.ResultsThe inner diameter of the left atrium was clearly enlarged in the atrial fibrillation group in comparison with that seen in the sinus rhythm group. The expression levels of both angiotensin II receptor type 1 mRNA and protein in the left atrial tissue were significantly increased in the patients with atrial fibrillation compared with those seen in patients with sinus rhythm (P < .05). Interestingly, the comparison of angiotensin II receptor type 2 expression levels in the left atrial tissue between these 2 groups is not statistically significant. In addition, the results of angiotensin II receptor type 1 or 2 expression in the right atrial tissue did not show any obvious change in the patients with atrial fibrillation versus those with sinus rhythm.ConclusionsExpression of angiotensin II receptor type 1 but not type 2 is highly upregulated only in the left atrial tissue of patients with rheumatic valvular disease with atrial fibrillation. This suggests that there is a possible pathophysiologic role of the renin–angiotensin system in patients with atrial fibrillation and that a series of effects mediated by the activation of angiotensin II receptor type 1 in the left atrial tissue might be one of the molecular mechanisms involved in the process of atrial remodeling in atrial fibrillation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|