β-lapachone and α-nor-lapachone modulate Candida albicans viability and virulence factors
| Autor: | Daniel Clemente de Moraes, Maristela Barbosa Portela, Marcia R. Pinto, José Alexandre Da Rocha Curvelo, R.M.A. Soares, Kelly C. G. de Moura, Camila A. dos Anjos |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Antifungal Agents Virulence Factors 030106 microbiology Hyphae Morphogenesis Virulence Virulence factor Microbiology Cell wall Mice 03 medical and health sciences Drug Resistance Fungal Candida albicans Animals Cytotoxic T cell biology Chemistry Biofilm biology.organism_classification Yeast RAW 264.7 Cells 030104 developmental biology Infectious Diseases Naphthoquinones |
| Zdroj: | Journal de Mycologie Médicale. 28:314-319 |
| ISSN: | 1156-5233 |
| Popis: | Background Candida albicans is the most important fungal pathogen that causes infections in humans, and the search for new therapeutic strategies for its treatment is essential. Objective The aim of this study was to evaluate the activity of seven naphthoquinones (β-lapachone, β-nor-lapachone, bromide-β-lapachone, hydroxy-β-lapachone, α-lapachone, α-nor-lapachone and α-xyloidone) on the growth of a fluconazole-resistant C. albicans oral clinical isolate and the effects of these compounds on the viability of mammalian cells, on yeast's morphogenesis, biofilm formation and cell wall mannoproteins availability. Results All the compounds were able to completely inhibit the yeast growth. β-lapachone and α-nor-lapachone were the less cytotoxic compounds against L929 and RAW 264.7 cells. At IC50, β-lapachone inhibited morphogenesis in 92%, while the treatment of yeast cells with α-nor-lapachone decreased yeast-to-hyphae transition in 42%. At 50 μg/ml, β-lapachone inhibited biofilm formation by 84%, whereas α-nor-lapachone reduced biofilm formation by 64%. The treatment of yeast cells with β-lapachone decreased cell wall mannoproteins availability in 28.5%, while α-nor-lapachone was not able to interfere on this virulence factor. Taken together, data show that β-lapachone and α-nor-lapachone exhibited in vitro cytotoxicity against a fluconazole-resistant C. albicans strain, thus demonstrating to be promising candidates to be used in the treatment of infections caused by this fungus. |
| Databáze: | OpenAIRE |
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