Prioritization of Contaminants of Emerging Concern in Wastewater Treatment Plant Discharges Using Chemical:Gene Interactions in Caged Fish
| Autor: | Michael D. Kahl, Matthew A. Weberg, Edward J. Perkins, Natàlia Garcia-Reyero, Tanwir Habib, Gerald T. Ankley, Barbara Lynn Escalon, Jenna E. Cavallin, Linnea M. Thomas, Kathleen M. Jensen, Megan N. Hughes, Daniel L. Villeneuve |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Estrone Cyprinidae 010501 environmental sciences Biology Wastewater 01 natural sciences Risk Assessment Article Biological pathway 03 medical and health sciences Gene expression Environmental Chemistry Animals Gene 0105 earth and related environmental sciences Mutagenicity Tests General Chemistry Contamination 030104 developmental biology Environmental chemistry Fish Sewage treatment Female Surface water Water Pollutants Chemical Environmental Monitoring |
| Zdroj: | Environmental sciencetechnology. 51(15) |
| ISSN: | 1520-5851 |
| Popis: | We examined whether contaminants present in surface waters could be prioritized for further assessment by linking the presence of specific chemicals to gene expression changes in exposed fish. Fathead minnows were deployed in cages for 2, 4, or 8 days at three locations near two different wastewater treatment plant discharge sites in the Saint Louis Bay, Duluth, MN and one upstream reference site. The biological impact of 51 chemicals detected in the surface water of 133 targeted chemicals was determined using biochemical endpoints, exposure activity ratios for biological and estrogenic responses, known chemical:gene interactions from biological pathways and knowledge bases, and analysis of the co-variance of ovary gene expression with surface water chemistry. Thirty-two chemicals were significantly linked by co-variance with expressed genes. No estrogenic impact on biochemical endpoints was observed in male or female minnows. However, bisphenol A (BPA) was identified by chemical:gene co-variation as the most impactful estrogenic chemical across all exposure sites. This was consistent with identification of estrogenic effects on gene expression, high BPA exposure activity ratios across all test sites, and historical analysis of the study area. Gene expression analysis also indicated the presence of nontargeted chemicals including chemotherapeutics consistent with a local hospital waste stream. Overall impacts on gene expression appeared to related to changes in treatment plant function during rain events. This approach appears useful in examining the impacts of complex mixtures on fish and offers a potential route in linking chemical exposure to adverse outcomes that may reduce population sustainability. |
| Databáze: | OpenAIRE |
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