RABiT-II-DCA: A Fully-automated Dicentric Chromosome Assay in Multiwell Plates
Autor: | Guy Garty, Mikhail Repin, Sergey Pampou, David J. Brenner, Ekaterina Royba, Charles Karan |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Biophysics
Large population Biology Article 030218 nuclear medicine & medical imaging 03 medical and health sciences Dicentric chromosome Automation 0302 clinical medicine Biodosimetry Healthy volunteers Humans Radiology Nuclear Medicine and imaging Radiometry In Situ Hybridization Fluorescence Chromosome Aberrations Radiation Radiation dose Robotics Healthy Volunteers Robotic systems Fully automated 030220 oncology & carcinogenesis Fish Radioactive Hazard Release Biomedical engineering |
Zdroj: | Radiat Res |
Popis: | We developed a fully-automated dicentric chromosome assay (DCA) in multiwell plates. All operations, from sample loading to chromosome scoring, are performed, without human intervention, by the second-generation Rapid Automated Biodosimetry Tool II (RABiT-II) robotic system, a plate imager and custom software, FluorQuantDic. The system requires small volumes of blood (30 µl per individual) to determine radiation dose received as a result of a radiation accident or terrorist attack. To visualize dicentrics in multiwell plates, we implemented a non-classical protocol for centromere FISH staining at 37°C. The RABiT-II performs rapid analysis of chromosomes after extracting them from metaphase cells. With the use of multiwell plates, many samples can be screened at the same time. Thus, the RABiT-II DCA provides an advantage during triage when risk-based stratification and medical management are required for a large population exposed to unknown levels of ionizing radiation. |
Databáze: | OpenAIRE |
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