FLO8 deletion leads to azole resistance by upregulating CDR1 and CDR2 in Candida albicans
| Autor: | Yue Zhao, Ling-Ning Meng, Ming-Jie Xiang, Ce Shi, Jin-Yan Liu, Fang Wu, Wen-Jing Li |
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| Rok vydání: | 2019 |
| Předmět: |
Azoles
Antifungal Agents Itraconazole Virulence Microbial Sensitivity Tests Protein Serine-Threonine Kinases Biology medicine.disease_cause Microbiology Agar dilution Fungal Proteins 03 medical and health sciences Drug Resistance Fungal Candida albicans medicine Molecular Biology 030304 developmental biology 0303 health sciences Mutation 030306 microbiology Broth microdilution Membrane Transport Proteins General Medicine biology.organism_classification Null allele Trans-Activators Gene Deletion Fluconazole medicine.drug |
| Zdroj: | Research in Microbiology. 170:272-279 |
| ISSN: | 0923-2508 |
| Popis: | Candida albicans has the ability to switch reversibly between budding yeast, filamentous, pseudohypha, and hyphal forms, a process in which the transcription factor Flo8 plays an important role. This ability is important for the virulence and pathogenicity of C. albicans. To determine whether Flo8 plays a role in the regulation of drug sensitivity, we constructed a FLO8 null mutant flo8/flo8 from the parental strain SN152 and a Flo8-overexpressing strain, flo8/flo8::FLO8. The susceptibility of the isolates to antifungal agents was then evaluated using the agar dilution and broth microdilution methods. Expression of drug resistance-related genes by the isolates was investigated by real-time PCR. The flo8/flo8 mutation isolates exhibited increased resistance to fluconazole, voriconazole, and itraconazole compared with the wild-type and drug sensitivity was restored by FLO8 overexpression (flo8/flo8∷FLO8). Of seven drug resistance-related genes, the FLO8 null mutation resulted in increased CDR1 and CDR2 expression (1.60-fold and 5.27-fold, respectively) compared with SN152, while FLO8 overexpression resulted in decreased CDR1 expression (0.63-fold). These results suggest that Flo8 is involved in the susceptibility of C. albicans to antifungal azoles, with FLO8 deletion leading to constitutive overexpression of CDR1 and CDR2 and resistance to antifungal azoles. |
| Databáze: | OpenAIRE |
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