Characterization of acute renal allograft rejection by human serum proteomic analysis
| Autor: | Hui Guo, Weina Zhang, Y Xu, Xing-Guang Lin, Lan-jun Cai, Hongmin Zhou, Ze-min Fang, Wentao He, Ke Wu, Zhen-long Luo, Zhong-hua Chen, Ying Gao |
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| Rok vydání: | 2009 |
| Předmět: |
Vitamin
Adult Graft Rejection Male Proteomics Serum Spectrometry Mass Electrospray Ionization Apolipoprotein B Adolescent Proteome Electrospray ionization Biomedical Engineering Renal function Biochemistry Biomaterials chemistry.chemical_compound Young Adult Genetics medicine Humans Electrophoresis Gel Two-Dimensional Protein precursor Volunteer Chromatography High Pressure Liquid Earth-Surface Processes biology Vitamin D-Binding Protein Middle Aged Trypsin Molecular biology Kidney Transplantation chemistry Case-Control Studies Renal allograft biology.protein Female Biomarkers medicine.drug |
| Zdroj: | Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 29(5) |
| ISSN: | 1672-0733 |
| Popis: | To identify acute renal allograft rejection biomarkers in human serum, two-dimensional differential in-gel electrophoresis (2-D DIGE) and reversed phase high-performance liquid chromatography (RP-HPLC) followed by electrospray ionization mass spectrometry (ESI-MS) were used. Serum samples from renal allograft patients and normal volunteers were divided into three groups: acute rejection (AR), stable renal function (SRF) and normal volunteer (N). Serum samples were firstly processed using Multiple Affinity Removal Column to selectively remove the highest abundance proteins. Differentially expressed proteins were analyzed using 2-D DIGE. These differential protein spots were excised, digested by trypsin, and identified by RP-HPLC-ESI/MS. Twenty-two differentially expressed proteins were identified in serum from AR group. These proteins included complement C9 precursor, apolipoprotein A-IV precursor, vitamin D-binding protein precursor, beta-2-glycoprotein 1 precursor, etc. Vitamin D-binding protein, one of these proteins, was confirmed by ELISA in the independent set of serum samples. In conclusion, the differentially expressed proteins as serum biomarker candidates may provide the basis of acute rejection noninvasive diagnosis. Confirmed vitamin D-binding protein may be one of serum biomarkers of acute rejection. Furthermore, it may provide great insights into understanding the mechanisms and potential treatment strategy of acute rejection. |
| Databáze: | OpenAIRE |
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