Genomic contacts reveal the control of sister chromosome decatenation in E. coli
| Autor: | Cockram C, Romain Koszul, Conin B, Ingrid Billault-Chaumartin, Sayyed He, Olivier Espéli |
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| Přispěvatelé: | Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Régulation spatiale des Génomes - Spatial Regulation of Genomes, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Collège Doctoral, Sorbonne Université (SU), Université de Lausanne = University of Lausanne (UNIL), University of Oxford, This research was supported by funding from the European Research Council under the 7th Framework Program (ERC Grant Agreement 771813 to R.K.) and ANR (HiResBac ANR-15-CE11-0023-03 to R.K. and O.E)., ANR-15-CE11-0023,HiResBaCS,Structure des chromosomes bactériens revélée a haute résolution(2015), European Project: 771813,ERC-2017-COG,SynarchiC(2018) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Genetics
0303 health sciences biology Topoisomerase IV [SDV]Life Sciences [q-bio] 030302 biochemistry & molecular biology Chromosome Locus (genetics) Genetic analysis Genome Chromosome segregation 03 medical and health sciences Catenation biology.protein Sister chromatids 030304 developmental biology |
| Zdroj: | Biorxiv bioRxiv |
| Popis: | In bacteria, chromosome segregation occurs progressively, from the origin to the terminus, a few minutes after the replication of each locus. In-between replication and segregation, sister loci are maintained in an apparent cohesive state by topological links. Whereas topoisomerase IV (Topo IV), the main bacteria decatenase, controls segregation, little is known regarding the influence of the cohesion step on chromosome folding. In this work, we investigated chromosome folding in cells with altered decatenation activities. Within minutes after Topo IV inactivation, a massive chromosome reorganization takes place, associated with increases in trans-contacts between catenated sister chromatids and in long-range cis-contacts between the terminus and distant loci on the genome. A genetic analysis of these signals allowed us to decipher specific roles for Topo IV and Topo III, an accessory decatenase. Moreover we revealed the role of MatP, the terminus macrodomain organizing system and MukB, the E. coli SMC in organizing sister chromatids tied by persistent catenation links. We propose that large-scale conformation changes observed in these conditions reveal a defective decatenation hub located in the terminus area. Altogether, our findings support a model of spatial and temporal partition of the tasks required for sister chromosome segregation. |
| Databáze: | OpenAIRE |
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