DNA damage of lymphocytes in experimental chronic renal failure: Beneficial effects of losartan
| Autor: | Viera Spustová, Rastislav Dzúrik, Pavol Blažíček, Katarína Šebeková, August Heidland, Zora Krivošíková, Mária Dušinská |
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| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty lymphocyte DNA Free Radicals DNA damage Lymphocyte uremic toxin Renal function DNA break medicine.disease_cause Receptor Angiotensin Type 2 Losartan Receptor Angiotensin Type 1 Nephropathy Angiotensin Receptor Antagonists chemistry.chemical_compound Internal medicine medicine Animals oxidative stress Lymphocytes Rats Wistar ablation nephropathy Kidney Chemistry kidney ablation medicine.disease Malondialdehyde Rats medicine.anatomical_structure Endocrinology Nephrology Creatinine Kidney Failure Chronic Reactive Oxygen Species Biomarkers Oxidative stress DNA Damage medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0085-2538 |
| DOI: | 10.1046/j.1523-1755.2001.59780212.x |
| Popis: | DNA damage of lymphocytes in experimental chronic renal failure: Beneficial effects of losartan.BackgroundKidney diseases are associated with the accumulation of various uremic toxins increasing the oxygen free radical (OFR) activity with a number of serious consequences. One of them could be the impairment of DNA stability with the increased formation of DNA breaks.MethodsThe study was performed in 4/6 kidney ablation rat nephropathy lasting for three months. The results of sham-operated (Sham), remnant kidney (Nx), and Nx treated by losartan (NxL) were compared.ResultsNx significantly increased blood pressure, plasma creatinine, urea, hippurate, malondialdehyde (MDA), lipofuscin (LF), and the number of DNA breaks of lymphocytes. Losartan decreased the rise of blood pressure and inhibited the rise of creatinine plasma concentration but not of other variables, while it markedly inhibited the number of DNA breaks (Sham 15.9 ± 1.1, Nx 54.5 ± 1.7,P < 0.001;Nx/Sham, NxL 23.3 ± 2.6P < 0.001, NxL/Sham andP < 0.001 NxL/Nx).ConclusionsThe 4/6 kidney ablation nephropathy increases the susceptibility of lymphocyte DNA to breaks, and losartan inhibits the number of breaks by a mechanism independent on glomerular filtration, accumulation of MDA or LF (markers of oxidative stress), and hippurate (marker of the accumulation of middle molecular substances). An independent mechanism, probably the interference with proliferation, is suggested. |
| Databáze: | OpenAIRE |
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