Genetic Diversity of Human Immunodeficiency Virus Type 1 Strains in Kinshasa, Zaire
| Autor: | Claudiu I. Bandea, Michael E. St. Louis, Gerald Schochetman, William L. Heyward, Gregg M. Orloff, Musey Kavuka, Chris Brown, Karen E. Potts, Chin-Yih Ou, Marcia L. Kalish, Nsuami Malanda |
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| Rok vydání: | 1993 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Immunology Population HIV Infections HIV Envelope Protein gp120 V3 loop Biology Genes env Virus law.invention law Virology Humans Amino Acid Sequence education Peptide sequence Polymerase chain reaction Genetics education.field_of_study Base Sequence Nucleic acid sequence Gene Products env Sequence Analysis DNA Peptide Fragments Infectious Diseases DNA Viral Democratic Republic of the Congo HIV-1 Female Primer (molecular biology) |
| Zdroj: | AIDS Research and Human Retroviruses. 9:613-618 |
| ISSN: | 1931-8405 0889-2229 |
| DOI: | 10.1089/aid.1993.9.613 |
| Popis: | The envelope (env) gene of human immunodeficiency virus type 1 (HIV-1) was amplified by polymerase chain reaction (PCR) from the peripheral blood mononuclear cells (PBMCs) of 14 HIV-1-infected women from Kinshasa, Zaire. Amplified DNA was directly sequenced with a primer specific for the HIV-1 env C2 region. The predicted amino acid sequences for the C2-V3 region for the 14 specimens are presented. The tetrapeptide sequence, GPGQ, located at the crown of the V3 loop, is conserved in all specimens. The same tetrapeptide sequence is present in the Zairian isolate MAL, but not in other published Zairian isolates (Z6, ELI, Z321, JY1, and NDK). Sequence comparison of the env C2-V3 region among the 14 specimens from Kinshasa revealed a 9-25% range of nucleotide divergence, with an average of 16%. Divergence between the 14 specimens and the Zairian isolates MAL, Z6, ELI, Z321, JY1, and NDK ranged from 13 to 31%. A range of 18-28% nucleotide sequence divergence was demonstrated between the 14 Kinshasa specimens and the North American isolate MN. These results demonstrate the importance of examining HIV-1 samples from diverse geographic origins in the development of effective HIV-1 vaccines. |
| Databáze: | OpenAIRE |
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