Transcriptomics analysis of early embryonic stem cell differentiation under osteoblast culture conditions: Applications for detection of developmental toxicity
| Autor: | Daniel T. Sloper, Amy L. Inselman, Tao Han, Gwenn E. Merry, J. Edward Fisher, James C. Fuscoe, Deborah K. Hansen, Xinrong Chen, Wafa Harrouk, Melissa S. Tassinari |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Developmental toxicity Biology Toxicology Bioinformatics Article Biological pathway Transcriptome Mice 03 medical and health sciences SOX2 Cadmium Compounds medicine Animals Cells Cultured Osteoblasts Sulfates Gene Expression Profiling Embryogenesis Gene Expression Regulation Developmental Cell Differentiation Mouse Embryonic Stem Cells Osteoblast Embryonic stem cell Cell biology RUNX2 030104 developmental biology medicine.anatomical_structure |
| Zdroj: | Reproductive Toxicology. 69:75-83 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2017.02.001 |
| Popis: | The mouse embryonic stem cell test (mEST) is a promising in vitro assay for predicting developmental toxicity. In the current study, early differentiation of D3 mouse embryonic stem cells (mESCs) under osteoblast culture conditions and embryotoxicity of cadmium sulfate were examined. D3 mESCs were exposed to cadmium sulfate for 24, 48 or 72 h, and whole genome transcriptional profiles were determined. The results indicate a track of differentiation was identified as mESCs differentiate. Biological processes that were associated with differentiation related genes included embryonic development and, specifically, skeletal system development. Cadmium sulfate inhibited mESC differentiation at all three time points. Functional pathway analysis indicated biological pathways affected included those related to skeletal development, renal and reproductive function. In summary, our results suggest that transcriptional profiles are a sensitive indicator of early mESC differentiation. Transcriptomics may improve the predictivity of the mEST by suggesting possible modes of action for tested chemicals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect