A New Human Somatic Stem Cell from Placental Cord Blood with Intrinsic Pluripotent Differentiation Potential

Autor: Niklas Feldhahn, Hans Werner Müller, Graça Almeida-Porada, Jizong Gao, Andreas Knipper, Jörg Bender, Markus Müschen, Claudia Rosenbaum, Judith A. Airey, Gesine Kögler, Özer Degistirici, Susanne Greschat, Esmail D. Zanjani, Peter Wernet, Arnold I. Caplan, Sandra Sensken, Johannes C. Fischer, Stefanie Liedtke, Evan Colletti, Thorsten Trapp, Rüdiger V. Sorg
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Pathology
Umbilical Veins
Time Factors
Cell Transplantation
Cellular differentiation
Placenta
Wistar
Cell Culture Techniques
Medical and Health Sciences
Hippocampus
Polymerase Chain Reaction
0302 clinical medicine
Leukocytes
Adipocytes
Immunology and Allergy
Developmental
Myocytes
Cardiac
CD45
Femur
0303 health sciences
education.field_of_study
Neurotransmitter Agents
Blotting
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells
Gene Expression Regulation
Developmental
Cell Differentiation
ex vivo expansion
Fetal Blood
Flow Cytometry
Cell biology
Haematopoiesis
Phenotype
030220 oncology & carcinogenesis
Cord blood
cord blood
Cord Blood Stem Cell Transplantation
Stem cell
Cardiac
Western
Cell Division
Adult stem cell
medicine.medical_specialty
Population
Mononuclear
Immunology
Blotting
Western
regenerative medicine
Biology
Article
Bone and Bones
Cell Line
Immunophenotyping
03 medical and health sciences
developmental potential
Albumins
medicine
Animals
Humans
Antigens
Rats
Wistar
education
030304 developmental biology
Myocytes
Osteoblasts
Sheep
Myocardium
Hematopoietic Stem Cells
Rats
Transplantation
Gene Expression Regulation
Leukocytes
Mononuclear
Leukocyte Common Antigens
Zdroj: The Journal of Experimental Medicine
The Journal of experimental medicine, vol 200, iss 2
ISSN: 1540-9538
0022-1007
Popis: Here a new, intrinsically pluripotent, CD45-negative population from human cord blood, termed unrestricted somatic stem cells (USSCs) is described. This rare population grows adherently and can be expanded to 1015 cells without losing pluripotency. In vitro USSCs showed homogeneous differentiation into osteoblasts, chondroblasts, adipocytes, and hematopoietic and neural cells including astrocytes and neurons that express neurofilament, sodium channel protein, and various neurotransmitter phenotypes. Stereotactic implantation of USSCs into intact adult rat brain revealed that human Tau-positive cells persisted for up to 3 mo and showed migratory activity and a typical neuron-like morphology. In vivo differentiation of USSCs along mesodermal and endodermal pathways was demonstrated in animal models. Bony reconstitution was observed after transplantation of USSC-loaded calcium phosphate cylinders in nude rat femurs. Chondrogenesis occurred after transplanting cell-loaded gelfoam sponges into nude mice. Transplantation of USSCs in a noninjury model, the preimmune fetal sheep, resulted in up to 5% human hematopoietic engraftment. More than 20% albumin-producing human parenchymal hepatic cells with absence of cell fusion and substantial numbers of human cardiomyocytes in both atria and ventricles of the sheep heart were detected many months after USSC transplantation. No tumor formation was observed in any of these animals.
Databáze: OpenAIRE
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