JMJD-1.2 controls multiple histone post-translational modifications in germ cells and protects the genome from replication stress
| Autor: | Pier Giorgio Amendola, Yvonne C. Lussi, Toshia R. Myers, Anna Elisabetta Salcini |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
DNA Replication
0301 basic medicine Genome instability Jumonji Domain-Containing Histone Demethylases DNA damage lcsh:Medicine medicine.disease_cause Article Genomic Instability Histones 03 medical and health sciences chemistry.chemical_compound Histone methylation medicine Animals lcsh:Science Caenorhabditis elegans Caenorhabditis elegans Proteins Mutation Multidisciplinary biology lcsh:R Chromatin Cell biology Protein Transport Germ Cells 030104 developmental biology Histone chemistry biology.protein Demethylase lcsh:Q Protein Processing Post-Translational DNA DNA Damage |
| Zdroj: | Scientific Reports Myers, T R, Amendola, P G, Lussi, Y C & Salcini, A E 2018, ' JMJD-1.2 controls multiple histone post-translational modifications in germ cells and protects the genome from replication stress ', Scientific Reports, vol. 8, 3765, pp. 1-11 . https://doi.org/10.1038/s41598-018-21914-9 Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-21914-9 |
| Popis: | Post-translational modifications of histones, constitutive components of chromatin, regulate chromatin compaction and control all DNA-based cellular processes. C. elegans JMJD-1.2, a member of the KDM7 family, is a demethylase active towards several lysine residues on Histone 3 (H3), but its contribution in regulating histone methylation in germ cells has not been fully investigated. Here, we show that jmjd-1.2 is expressed abundantly in the germline where it controls the level of histone 3 lysine 9, lysine 23 and lysine 27 di-methylation (H3K9/K23/K27me2) both in mitotic and meiotic cells. Loss of jmjd-1.2 is not associated with major defects in the germ cells in animals grown under normal conditions or after DNA damage induced by UV or ionizing irradiation. However, jmjd-1.2 mutants are more sensitive to replication stress and the progeny of mutant animals exposed to hydroxyurea show increased embryonic lethality and mutational rate, compared to wild-type. Thus, our results suggest a role for jmjd-1.2 in the maintenance of genome integrity after replication stress and emphasize the relevance of the regulation of histone methylation in genomic stability. |
| Databáze: | OpenAIRE |
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