Effects of a 50 Hz sinusoidal magnetic field on cell adhesion molecule expression in two human osteosarcoma cell lines (MG-63 and Saos-2)
| Autor: | Pietro L. Indovina, Gianfranco Donelli, Gabriella Rainaldi, Maria Teresa Santini, Paolo Vecchia, Antonella Ferrante |
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| Přispěvatelé: | Santini, M. T., Rainaldi, G., Ferrante, A., Indovina, PIETRO LUIGI MARIA, Vecchia, P., Donelli, G. |
| Rok vydání: | 2003 |
| Předmět: |
Physiology
Integrin Biophysics Apoptosis Radiation Dosage Cell cycle phase Electromagnetic Fields Electricity Cell Line Tumor Humans Radiology Nuclear Medicine and imaging Cell Size Osteosarcoma biology Cell growth Cell adhesion molecule CD44 Dose-Response Relationship Radiation General Medicine Cell cycle Cell biology Gene Expression Regulation Neoplastic Fibronectin Hyaluronan Receptors biology.protein Neural cell adhesion molecule Integrin alpha2beta1 Cell Adhesion Molecules Cell Division Integrin alpha5beta1 |
| Zdroj: | Bioelectromagnetics. 24:327-338 |
| ISSN: | 1521-186X 0197-8462 |
| Popis: | The possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 0.5 mT can induce variations in the expression of cell adhesion molecules (CAMs) in two human osteosarcoma cell lines (MG-63 and Saos-2) was investigated. In particular, the expression of two important integrins, VLA-2, the receptor for collagen, and VLA-5, the receptor for fibronectin, as well as CD44, were examined in both cell lines after these had been exposed for 7 and 14 days to a 50 Hz, 0.5 mT field. Cell surface morphology (scanning electron microscopy), cell growth characteristics (growth curves and cell cycle phase distribution), and cell death (necrosis and apoptosis) were also examined. The results demonstrate that no variations in surface morphology and cell death occurred between control and exposed cells in both MG-63 and Saos-2 cells, while significant changes were noted in cell growth and fibronectin and CD44 expression in MG-63 cells. The results are discussed in view of the important role that CAMs play in controlling various cancer cell functions, particularly proliferation and metastasis. |
| Databáze: | OpenAIRE |
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