Enhanced myeloid specificity of CD 117 compared with CD13 and CD33
| Autor: | Olga Lopez, E Rubiol, Josep F. Nomdedeu, Josep Ubeda, Carme Rio, Andreu Llorente, Camino Estivill, R Mateu, Albert Altés |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Cancer Research Myeloid Adolescent Sialic Acid Binding Ig-like Lectin 3 CD33 Antigens Differentiation Myelomonocytic CD13 Antigens Proto-Oncogene Mas Immunophenotyping Diagnosis Differential Myelogenous Antigens CD hemic and lymphatic diseases Acute lymphocytic leukemia medicine Humans Cell Lineage Child Aged Aged 80 and over Acute leukemia ABL business.industry Myelodysplastic syndromes Infant Newborn Infant Hematology Middle Aged Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease digestive system diseases Proto-Oncogene Proteins c-kit Leukemia medicine.anatomical_structure Oncology Leukemia Myeloid Child Preschool Myelodysplastic Syndromes Acute Disease Immunology business Biomarkers |
| Zdroj: | Leukemia Research. 23:341-347 |
| ISSN: | 0145-2126 |
| DOI: | 10.1016/s0145-2126(98)00185-4 |
| Popis: | The c-kit proto-oncogene encodes a 145 kd tyrosine kinase transmembrane receptor, which plays a key role in haemopoiesis. The c-kit has been classified as CD117 and is especially useful in the differential diagnosis of acute myelogenous leukemia (AML) and acute lymphoblastic leukemia (ALL). We analysed 104 consecutive cases (55 AML, 23 B-cell lineage ALL, three T-cell ALL, 11 blast crisis of chronic myeloproliferative disorders and 12 cases of myelodysplastic syndromes with more than 10% of blasts) referred to our Hospital for immunophenotypic diagnosis and compared the expression pattern of CD13, CD33 and CD117 using the same fluorochrome (phycoerythrin-PE). The recommendations of the EGIL group were followed in order to establish lineage involvement of the blastic population. The threshold used to assign positivity for CD117 was 10%. Bcr/abl, TEL/AML-1 and MLL rearrangements were assessed by molecular methods. CD117 expression was detected in 91% of AML and MDS. All the negative cases corresponded to acute monocytic leukemias. The calculated specificity for myeloid involvement was 0.86 for CD117, 0.36 for CD13 and 0.44 for CD33 (P < 0.005). CD117 was also positive in four cases of ALL. None of these cases showed bcr/abl or MLL rearrangements. In the light of these findings, CD117 expression should yield a higher score, at least one point, in the system currently applied for the diagnosis of biphenotypic acute leukemias (BAL) as its myeloid specificity is greater than that of CD13 and CD33. Moreover, its absence in AML could identify two subgroups of M5b cases. The coexpression of CD117 with cytoplasmic CD79a is often associated with CD7 reactivity, suggesting a stem cell disorder. CD117 should be included on a routine basis for the immunophenotypic diagnosis of acute leukemias. |
| Databáze: | OpenAIRE |
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