Evaluation of Carbodiimides Using a Competition Method
| Autor: | Jan Izdebski, Danuta Kunce |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Pharmacology
chemistry.chemical_classification Alanine Phenylalanine Organic Chemistry Peptide General Medicine Biochemistry Carbodiimides chemistry.chemical_compound Dicyclohexylcarbodiimide chemistry Leucine Structural Biology Reagent Drug Discovery Molecular Medicine Organic chemistry Peptide bond Aminoacylation Indicators and Reagents Peptides Molecular Biology Resins Plant Carbodiimide |
| Zdroj: | Journal of Peptide Science. 3:141-144 |
| ISSN: | 1099-1387 1075-2617 |
| DOI: | 10.1002/(sici)1099-1387(199703)3:2<141::aid-psc93>3.0.co;2-a |
| Popis: | A competitive reaction of activated Boc-Ala-OH and Boc-Phe-OH with H-Leu-resin has been developed for assessing the relative efficiencies of different carbodiimides. This allowed a comparison of the efficiency of the carbodiimides N,N;′-dicyclohexylcarbodiimide,N,N′-diisopropylcarbodiimide, N-tert-butyl-N′-methylcarbodiimide and N-tert-butyl-N′-ethylcarbodiimide. Comparable results were obtained when these reagents were used for the preformation of symmetrical anhydrides or of 1-hydroxybenzotriazole esters in situ. Differential incorporation was observed when asymmetrical carbodiimides were used for peptide bond formation by the direct carbodiimide procedure. © 1997 European Peptide Society and John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text