Differential effect on different immune subsets of neoadjuvant chemotherapy in patients with TNBC
| Autor: | Chiara Massa, Christian Schem, Jens Huober, Carsten Denkert, Christian Jackisch, Michael Untch, Thomas Karn, Marion van Mackelenbergh, Anja Mueller, Claus Hanusch, Volkmar Müller, Sibylle Loibl, Andreas Schneeweiss, Elmar Stickeler, Barbara Seliger, Peter A. Fasching, Karsten Weber, Jörg Thomalla, Jens-Uwe Blohmer, Katharina Biehl, Frederik Marmé, Dirk-Michael Zahm |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Cancer Research Cyclophosphamide medicine.medical_treatment Immunology Triple Negative Breast Neoplasms Breast cancer Immune system breast neoplasms medicine Humans Immunology and Allergy RC254-282 Aged Clinical/Translational Cancer Immunotherapy Pharmacology immunocompetence Chemotherapy business.industry Tumor-infiltrating lymphocytes Neoplasms. Tumors. Oncology. Including cancer and carcinogens Immunotherapy Middle Aged medicine.disease Neoadjuvant Therapy Oncology Cancer research Molecular Medicine Female business CD8 Epirubicin medicine.drug |
| Zdroj: | Journal for ImmunoTherapy of Cancer, Vol 8, Iss 2 (2020) Journal for Immunotherapy of Cancer |
| ISSN: | 2051-1426 |
| Popis: | BackgroundTriple-negative breast cancer (TNBC) is the most aggressive form of breast cancer (BC). Due to the absence of targets such as HER2 or hormone receptors, early TNBC is treated with surgery and chemotherapy. Since TNBC is also considered the most immunogenic type of BC with tumor infiltrating lymphocytes that are predictive for chemotherapy response and prognostic for patients′ survival, many different immunotherapeutic strategies are currently explored in clinical trials for the treatment of this disease. In order to efficiently combine chemotherapy with immunotherapy, it is important to evaluate the effect of chemotherapy on immune cells in vivo.MethodsPeripheral blood was taken from 56 patients with TNBC undergoing neoadjuvant chemotherapy with nanoparticle albumin-bound paclitaxel (Nab-Pac) followed by epirubicin and cyclophosphamide (EC) at three different time points. Multicolor flow cytometry was used to characterize the immune cell composition and functional properties along neoadjuvant chemotherapy.ResultsWhereas the first phase of the neoadjuvant chemotherapy did not significantly alter the patients′ immune cell composition, after the second phase of chemotherapeutic administration most B cells (>90%) were lost and the frequency of natural killer (NK) cells and CD4+ T lymphocytes decreased approximately to 50%. In contrast, the frequency of CD8+ T cells were less affected.ConclusionsDespite late consequences of Nab-Pac cannot be ruled out, these data suggest that different chemotherapeutics might have distinct effects on the immune cell repertoire and that different immune cell populations exhibit a specific susceptibility to these chemotherapies with B and NK cells being more affected than T cells. This might also have an impact on the combination of chemotherapies with immunotherapies.Trial registration numberNCT02685059. |
| Databáze: | OpenAIRE |
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