JoVE Methods Collection Highlights: Protein-Protein Interactions

Autor: Michael Overduin, Alastair J. Barr
Rok vydání: 2019
Předmět:
Zdroj: Journal of visualized experiments : JoVE. (148)
ISSN: 1940-087X
Popis: Protein-protein interactions (PPIs) are fundamental to the generation of biological effects. Many are intimately linked with disease, and they are increasingly recognized as offering important targets for drug action1. Numerous techniques are available to study these protein interactions, some of which are discussed in this collection or in other JoVE articles. Each technique has its own merits and limitations2; however, there is no one perfect technique for all PPIs due to the massive diversity of these interactions which occur in different subcellular compartments, with a wide range of affinities (picomolar to millimolar), and may be only transient in nature. In vitro kinase assays are an indirect approach for studying PPIs that can be used to analyze how kinases perform their functional role of transferring a phosphate group from ATP to a substrate. Cui et al.3 use this technique, coupled with tryptic digest and mass spectrometry, to identify the cyclin-dependent kinase-1-specific phosphorylation sites on a fragment of the human centromere protein F (CENP-F). For functional verification of novel phosphorylation sites, the authors use a binding assay, combining CENP-F containing a phosphomimetic mutation and karyopherin α, a nuclear transport receptor, thus providing cross-validation. Coimmunoprecipitation is a widely used method for studying PPIs in which an antibody is used to capture a specific target together with any other protein molecules that are associated with it. Zheng and colleagues4 apply this technique to study hypoxia-inducible factors (HIFs) that function as heterodimers composed of an oxygen-regulated α subunit and a constitutively expressed β subunit also known as ARNT3. An interesting note about their protocol is that the cells are induced and harvested under hypoxic conditions, and endogenous proteins from nuclear fractions are then coimmunoprecipitated. As the authors note, one limitation of the method is that it cannot determine if PPIs are direct or indirect. Nolan et al.5 present a protocol for the measurement of the number of molecules and their brightness in fluorescence microscopy images 'Number and Brightness' (NB it is a three-in-one extraction protocol that enables both PPIs and PMIs to be studied and applies affinity purification to the planting of cells. The proximity ligation assay has the advantage of being used to detect interactions between endogenous proteins in cells and tissues. Karchugina and Chernoff7 use the technique to show the presence of heterodimers of the kinases MST1 and MST2 in human Schwann cells (HSCs) and human embryonic kidney cells (HEK-293). Their article has several valuable pointers on how to perform the technique successfully. Appropriate primary antibodies raised in different species against each interacting protein are required, and since the specificity and sensitivity of the antibodies are key, antibody concentrations should be finely tuned. The authors found glass chamber slides convenient for analyzing multiple cell lines and antibody combinations but noted that it is imperative to remove all of the silicone insert between wells to avoid differences in the confocal distance during microscopy. In addition, various positive and negative controls are used to validate the results. Nuclear magnetic resonance (NMR) spectroscopy is a sensitive assay that can be used to provide atomic-level resolution and quantitative information about protein interactions and protein-ligand interactions. Winkelaar and colleagues8 use the technique to study the interaction of two proteins (vimentin and envoplakin) found in desmosomes, cell structures involved in cell-to-cell adhesion that are typically found in tissues that experience intense mechanical stress. The authors use a 15N-labelled vimentin domain and acquire a 2D spectrum using heteronuclear single quantum correlation (HSQC). In the presence of a domain from envoplakin, extensive line broadening and peak disappearance are observed, consistent with protein interaction. The authors further probe the basis of the interaction using mutagenesis and microscale thermophoresis (MST) to provide quantitative information. Small-angle X-ray scattering (SAXS) is a technique that can be used to provide information on the size and conformations of a macromolecule in solution and generate a molecular envelope of large, multidomain or protein complexes at low resolution. In the protocol by Mrozowich et al.9, the technique is used to generate a low-resolution structure of nidogen-1 and laminin γ-1 in complex. The technique may be of interest to researchers studying PPIs in large proteins with multiple globular domains that are too big for NMR spectroscopy and are challenging to crystallize for X-ray crystallography. It has been estimated there are some 650,000 interactions in the human interactome10-enough to keep researchers in the field busy for some time to come. A recent development, made possible by advances in microscopy, mass spectrometry, and machine learning, is spatial proteomics that may provide great insight in this area in the future11. This collection of PPI methods will help researchers tackle this important challenge while avoiding potential pitfalls.
Databáze: OpenAIRE
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