Systems Level Insights Into the Stress Response to UV Radiation in the Halophilic ArchaeonHalobacterium NRC-1
| Autor: | Jocelyne DiRuggiero, Nitin S. Baliga, Chika Iloanusi, Min Pan, Sarah J. Bjork, Leroy Hood, Richard Bonneau, Molly C. Kottemann |
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| Rok vydání: | 2004 |
| Předmět: |
Halobacterium
Time Factors DNA Repair Light Ultraviolet Rays DNA repair Archaeal Proteins RNA Archaeal Computational biology medicine.disease_cause Mice Cricetinae Sequence Homology Nucleic Acid Genetics medicine Animals RNA Messenger Letters Photolyase Gene Genetics (clinical) Gene knockout Comparative genomics Mutation Mesocricetus biology Gene Expression Profiling biology.organism_classification Repressor Proteins Survival Rate DNA mismatch repair Gene Expression Regulation Archaeal Deoxyribodipyrimidine Photo-Lyase Transcription Factors |
| Zdroj: | Genome Research. 14:1025-1035 |
| ISSN: | 1088-9051 |
| Popis: | We report a remarkably high UV-radiation resistance in the extremely halophilic archaeonHalobacterium NRC-1withstanding up to 110 J/m2with no loss of viability. Gene knockout analysis in two putative photolyase-like genes (phr1andphr2) implicated onlyphr2in photoreactivation. The UV-response was further characterized by analyzing simultaneously, along with gene function and protein interactions inferred through comparative genomics approaches, mRNA changes for all 2400 genes during light and dark repair. In addition to photoreactivation, three other putative repair mechanisms were identified including d(CTAG) methylation-directed mismatch repair, four oxidative damage repair enzymes, and two proteases for eliminating damaged proteins. Moreover, a UV-induced down-regulation of many important metabolic functions was observed during light repair and seems to be a phenomenon shared by all three domains of life. The systems analysis has facilitated the assignment of putative functions to 26 of 33 key proteins in the UV response through sequence-based methods and/or similarities of their predicted three-dimensional structures to known structures in the PDB. Finally, the systems analysis has raised, through the integration of experimentally determined and computationally inferred data, many experimentally testable hypotheses that describe the metabolic and regulatory networks ofHalobacterium NRC-1. |
| Databáze: | OpenAIRE |
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