Effects of Water-Soluble Polysaccharides with Different Chemical Structure, Isolated from Acorus calamus L. and Trifolium pratense L., on Nitric Oxide Production: A Screening Study
| Autor: | M. V. Belousov, V. V. Zhdanov, A. M. Guriev, E. S. Trofimova, Mekhman S. Yusubov, A. A. Ligacheva, E. Yu. Sherstoboev, A. P. Korzh, A. M. Dygai, S. V. Krivoshchekov, M. G. Danilets |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
0301 basic medicine
Male Chemical structure Ion chromatography Polysaccharide Nitric Oxide General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Mice Polysaccharides Botany Acorus calamus Acorus Animals Food science Nitrite Cells Cultured chemistry.chemical_classification biology Macrophages General Medicine biology.organism_classification Rhizome 030104 developmental biology chemistry Pectins Composition (visual arts) Female Trifolium |
| Zdroj: | Bulletin of experimental biology and medicine. 160(3) |
| ISSN: | 1573-8221 |
| Popis: | Screening study of the effects of sweet flag (Acorus calamus L.) rhizome and clover (Trifolium pratense L.) aerial part on the production of NO by mouse macrophages was carried out. The polysaccharides were separated by ion exchange chromatography into fractions differing by monomeric composition and ramification type and were used in concentrations of 20, 40, and 100 μg/ml. Four fractions of Acorus calamus L. (PSF-101, PSF-102, PSF-103, and PSF-105), used in different concentrations, moderately stimulated nitrite production by macrophages. Three of five Trifolium pratense L. polysaccharides (PS62-3, PS62-4, and PS62-5) exhibited a significant specific effect on NO production. Rhamnogalactouronans from clover PS63-3 in all concentrations and from PS62-5 in a concentration of 100 μg/ml exhibited the highest activity, comparable to the NO-stimulatory activity of the reference LPS, while polysaccharide PS62-3 in a concentration of 40 μg/ml exhibited even higher activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink