Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
| Autor: | J. M. Besterman, P. P. Leitner, Ana María Cortizo, K. A. Chandrabose |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Agonist
medicine.drug_class Prostaglandin Riñón Biology Bradykinin Kidney Biochemistry Phospholipases A eicosanoids Cell Line Diffusion chemistry.chemical_compound Adenosine Triphosphate Dogs Endocrinology Phospholipase A2 medicine Extracellular Animals Trypsin Arachidonic Acid Tight junction Ionomycin Thrombin Ciencias Químicas Receptor-mediated endocytosis Cell biology Madin-Darby canine kidney cells Kinetics Phospholipases A2 Eicosanoid chemistry Cell culture Ciencias Médicas Prostaglandins biology.protein phospholipase A2 Chromatography Thin Layer phospholipase A2 eicosanoids Madin-Darby canine kidney cells |
| Zdroj: | CIC Digital (CICBA) Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| Popis: | The receptor mediated activation of phospholipase A2by appropriate ligands results in the synthesis and release of eicosanoids, a class of potent bioregulatory molecules. Madin-Darby canine kidney cells (MDCK) are polarized epithelial cells, with structurally and functionally distinct plasma membrane domains separated by tight junctions. Using MDCK cells grown in dual sided chambers, we show in this report, that a) the receptor mediated release of prostaglandins and arachidonate into the extracellular medium is predominantly unidirectional, b) the direction of release is agonist specific, and c) the magnitude of the response due to a given agonist is cell-domain specific. These characteristics, if operativein vivo, would contribute towards the optimal function of trans-cellular metabolism of eicosanoids already demonstrated. Facultad de Ciencias Médicas |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|