In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites
| Autor: | Luís César de Castro, Carla Kauffmann, Eduardo Miranda Ethur, Rodrigo Dall'Agnol, Paulo Michel Roehe, Ismael Pretto Sauter, ST Vand Der Sand, Juliana de Souza, Luciana Weidlich, José Carlos Germani, Paula Michele Lohmann, Aleksander Westphal Muniz, Samuel Paulo Cibulski, Marilise Brittes Rott |
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| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
biology Acanthospermum australe fungi Acanthamoeba polyphaga lcsh:RS1-441 Asteraceae biology.organism_classification medicine.disease In vitro lcsh:QK1-989 Acanthamoeba Microbiology lcsh:Pharmacy and materia medica Complementary and alternative medicine Acanthamoeba keratitis lcsh:Botany parasitic diseases medicine atividade amebicida Cytotoxicity Medicinal plants Polyphaga |
| Zdroj: | Revista Brasileira de Plantas Medicinais, Vol 15, Iss 4, Pp 589-594 (2013) |
| ISSN: | 1516-0572 |
| DOI: | 10.1590/s1516-05722013000400016 |
| Popis: | Acanthamoeba is a free-living protozoan widely distributed in the environment, occurring in vegetative trophozoite and resistance cyst stages during its life cycle. It constitutes an etiological agent of Acanthamoeba keratitis, a disease that may cause severe ocular inflammation and blindness. New drugs can be developed from molecules found in plants and thus help in its difficult treatment. Acanthospermum australe (Asteraceae), a plant used in folk medicine, had its effect tested on Acanthamoeba polyphaga. Aqueous and ethanolic extracts of A. austral were obtained from aerial parts for infusion and static maceration, respectively. Concentrations of 10, 5, 2.5, 1.25 and 0.625 mg/ml of the extract were tested against Acanthamoeba polyphaga trophozoites. The cytotoxic effect of the extracts was tested in mammalian cells using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The 10 mg/ml concentration of ethanolic extract was lethal to 100% of the A. polyphaga trophozoites in 24 h and both extracts presented cytotoxic effect against mammalian cells. These findings suggest that the A. austral ethanolic extract may have compounds with relevance to the development of new amoebicidal drugs. |
| Databáze: | OpenAIRE |
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