Characterization of a spermine/spermidine transport system reveals a novel DNA sequence duplication in Neisseria gonorrhoeae
| Autor: | Sandeep J. Joseph, Maira Goytia, Vijaya Dhulipala, Timothy D. Read, Leo Hawel, William M. Shafer |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Sexually transmitted disease
Spermidine transport Operon Spermidine Molecular Sequence Data Spermine Biology medicine.disease_cause Microbiology chemistry.chemical_compound Gene Duplication Genetics medicine Research Letter Amino Acid Sequence Molecular Biology Gene Polyamine transport Base Sequence Computational Biology Membrane Transport Proteins Biological Transport Molecular biology Neisseria gonorrhoeae Culture Media chemistry Mutation |
| Popis: | During infection, Neisseria gonorrhoeae, the causative agent of the sexually transmitted disease gonorrhea, comes into contact with numerous host compounds including polyamines (e.g. spermine and spermidine). Here, we show that spermine and spermidine concentrations in the growth medium decrease to undetectable levels in the presence of gonococci over time, but not when proteins of the putative polyamine transport system are lost due to mutation. We propose that gonococci have a functional and sole polyamine transport system (PotFGHI) that specifically imports spermine and spermidine. Bioinformatics and molecular analyses showed that the transporter's potGHI genes are organized as an operon while the gene encoding the necessary cognate periplasmic polyamine-binding protein (PotF) is located elsewhere on the chromosome. Interestingly, within the potGHI locus, we identified a novel duplicated sequence, which we term the Pot-Gene-Associated-Duplication-Element, present in variable copy numbers in different gonococcal strains that was likely formed from the 5(') and 3(') ends of the coding sequences of the tandemly linked potH and potG genes, respectively. |
| Databáze: | OpenAIRE |
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