The carry-over of Campylobacter isolates between sequential poultry flocks
Autor: | J. E. Shreeve, Diane G. Newell, Toszeghy M, A Ridley |
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Rok vydání: | 2002 |
Předmět: |
Veterinary medicine
Genotype animal diseases medicine.disease_cause Campylobacter jejuni law.invention Microbiology Restriction fragment Food Animals law Campylobacter Infections Pulsed-field gel electrophoresis medicine Disease Transmission Infectious Animals Typing Animal Husbandry Serotyping Polymerase chain reaction Poultry Diseases Disease Reservoirs Molecular Epidemiology General Immunology and Microbiology biology Campylobacter biology.organism_classification United Kingdom Electrophoresis Gel Pulsed-Field biology.protein Animal Science and Zoology Flock Chickens Polymorphism Restriction Fragment Length Flagellin |
Zdroj: | Avian diseases. 46(2) |
ISSN: | 0005-2086 |
Popis: | The carry-over of Campylobacter strains from one flock to a subsequent flock in the same broiler house has been studied using molecular epidemiological techniques. In all, 524 Campylobacter strains, isolated from two sequential broiler flocks from 60 broiler houses, were typed by restriction fragment polymorphism of the polymerase chain reaction (PCR) product of the flaA and flaB genes (fla typing). Selected strains were also typed using pulsed field gel electrophoresis (PFGE). By fla typing, 15 (21%) of the 60 houses with Campylobacter-positive sequential flocks had identical genotypes. In 10 (16% overall) of these houses the strains were also identical by PFGE. The difference in PFGE patterns in the strains from the three remaining houses may be indicative of genetic instability. Overall, these results suggest that carry-over from one flock to a subsequent flock in the same house is a relatively infrequent event and, therefore, that routine broiler house cleansing and/or disinfection is largely adequate to eliminate Campylobacter contamination. An alternative explanation of the low level carry-over is a persistent source or reservoir, external to the environment of the broiler houses. |
Databáze: | OpenAIRE |
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