Identification of cytochrome P450 2E1 in rat brain
| Autor: | Min Yoo, Kwan-Hee You, Hye-Myung Ryu, Chul-Ho Yun, Young-Mi Ji, Soon Chul Lee, Song-Woo Shin |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Messenger RNA DNA Complementary Pcr cloning Biophysics Brain Cytochrome P-450 CYP2E1 Cell Biology CYP2E1 Biology Rat brain Biochemistry Molecular biology Polymerase Chain Reaction Rats Rats Sprague-Dawley Blotting Southern Complementary DNA Rat liver Animals RNA Messenger Molecular Biology Pcr analysis Southern blot |
| Zdroj: | Biochemical and biophysical research communications. 231(2) |
| ISSN: | 0006-291X |
| Popis: | We have isolated cDNA fragments that were originated from P450 2E1 in rat brain by PCR analysis. Their size matched up to what we expected based on the reported sequence of rat liver P450 2E1 mRNA. Internal structure of the longest first-round PCR products were investigated by Southern blot analysis and “nested” PCR. Their results confirmed that PCR products actually originated from P450 2E1 mRNA in rat brain. RT-PCR was also carried out using P450 2E1 specific primers and the size of the product was exactly as we expected for P450 2E1. These experimental evidences should clarify the presence of P450 2E1 in rat brain. |
| Databáze: | OpenAIRE |
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