Distinct neuronal populations in the basolateral and central amygdala are activated with acute pain, conditioned fear, and fear-conditioned analgesia
| Autor: | Sarah Ehling, Mary A. Hughes, William C. Wetsel, Dipendra K. Aryal, Megan Barbar, Charles E. Smith, B. Duncan X. Lascelles, Jess Thomas, Ryan K. Butler, Vladimir M. Pogorelov |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine endocrine system Inhibitory postsynaptic potential Amygdala 03 medical and health sciences 0302 clinical medicine Ca2+/calmodulin-dependent protein kinase Conditioning Psychological medicine Animals Pain Management Periaqueductal Gray Neurons Fear processing in the brain biology General Neuroscience Central Amygdaloid Nucleus Fear Acute Pain Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Nociception nervous system biology.protein GABAergic Analgesia NeuN Psychology Neuroscience 030217 neurology & neurosurgery Parvalbumin |
| Zdroj: | Neuroscience Letters. 661:11-17 |
| ISSN: | 0304-3940 |
| Popis: | Fear-conditioned analgesia (FCA) is modulated by brain areas involved in the descending inhibitory pain pathway such as the basolateral (BLA) and central amygdala (CEA). The BLA contains Ca2+/calmodulin-dependent protein kinase II (CaMKII) and parvalbumin (PV) neurons. CEA neurons are primarily inhibitory (GABAergic) that comprise enkephalin (ENK) interneurons and corticotropin-releasing factor (CRF) - neurons that project to the periaqueductal grey. The purpose of our experiment was to determine the pattern of activation of CaMKII/PV and ENK/CRF neurons following the expression of acute pain, conditioned fear, and FCA. A significant reduction was observed in nociceptive behaviors in mice re-exposed to a contextually-aversive environment. Using NeuN and cFos as markers for activated neurons, CaMKII, PV, ENK, or CRF were used to identify neuronal subtypes. We find that mice expressing conditioned fear displayed an increase in c-Fos/CaMKII co-localization in the lateral amygdala and BLA compared to controls. Additionally a significant increase in cFos/CRF co-localization was observed in mice expressing FCA. These results show that amygdala processing of conditioned contextual aversive, nociceptive, and FCA behaviors involve different neuronal phenotypes and neural circuits between, within, and from various amygdala nuclei. This information will be important in developing novel therapies for treating pain and emotive disorders in humans. |
| Databáze: | OpenAIRE |
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