Clinical Usefulness of Cell-Free DNA as a Prognostic Marker in Acute Ischemic Stroke
| Autor: | Onjal Taywade, Atulabh Vajpeyee, Teddy Wijatmiko, Swapnil Pandey, Puneet Singh Chauhan, Manisha Vajpeyee |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_specialty Stroke patient 030204 cardiovascular system & hematology Brain Ischemia 03 medical and health sciences 0302 clinical medicine Modified Rankin Scale Internal medicine Medicine Humans Acute ischemic stroke Stroke Aged Aged 80 and over business.industry Stroke scale General Medicine Middle Aged medicine.disease Prognosis Mechanical thrombectomy Real-time polymerase chain reaction Cell-free fetal DNA Female Neurology (clinical) business Cell-Free Nucleic Acids 030217 neurology & neurosurgery Biomarkers |
| Zdroj: | The neurologist. 25(1) |
| ISSN: | 2331-2637 |
| Popis: | OBJECTIVE Stroke has become the second most common cause of death. Several biomarkers have been detected in the peripheral blood from stroke patients, but none has found a place in clinical practice. Cell-free DNA (cf-DNA) liberated into the blood soon after the onset of stroke might be useful for assessing disease severity and prognosis. STUDY DESIGN AND METHODS A total of 54 patients presenting with ischemic stroke were recruited consecutively with the exclusion of patients having trauma, tumor, infections, and organ failure. The cf-DNA was extracted by circulating nucleic acid kit from Qiagen and measured by real-time quantitative polymerase chain reaction assay for β-globin gene. The primary outcome measure was poststroke modified Rankin scale Score at 3 months after the onset of symptoms. RESULTS Higher cf-DNA levels were associated with severity at the time of admission measured by National Institutes of Health Stroke Scale (P=0.003) and poor outcome as measured by modified Rankin scale 3-month scores (P=0.001). Therapeutic intervention in the form of a mechanical thrombectomy or IV thrombolysis was associated with improved outcome in patients with cf-DNA |
| Databáze: | OpenAIRE |
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