Morphofunctional characteristics of skeletal muscle in rats with cerebral palsy

Autor:	Caroline Covatti, Márcia Miranda Torrejais, Lígia Aline Centenaro, Rose Meire Costa Brancalhão, Pâmela Buratti
Rok vydání:	2019
Předmět:	Lipopolysaccharides Male medicine.medical_specialty medicine.medical_treatment Neuromuscular Junction Motor Activity Body weight Neuromuscular junction Pathology and Forensic Medicine Cerebral palsy Pregnancy Trichrome Internal medicine medicine Animals Rats Wistar Hypoxia Muscle Skeletal Molecular Biology Saline business.industry Cerebral Palsy Skeletal muscle Original Articles Cell Biology medicine.disease Disease Models Animal Endocrinology medicine.anatomical_structure Female Collagen Plantaris muscle business Locomotion
Zdroj:	International Journal of Experimental Pathology. 100:49-59
ISSN:	1365-2613 0959-9673
DOI:	10.1111/iep.12304
Popis:	Knowledge of skeletal muscle adaptations is important to understand the functional deficits in cerebral palsy (CP). This study aimed to investigate the morphofunctional characteristics of skeletal muscle in a CP animal model. Initially, pregnant Wistar rats were injected intraperitoneally with saline or lipopolysaccharide over the last five days of pregnancy. The control group (n = 8) consisted of male pups born to females injected with saline. The CP group (n = 8) consisted of male pups born to females injected with lipopolysaccharide, which were submitted to perinatal anoxia [day of birth, postnatal day 0 (P0)] and sensorimotor restriction (P1‐P30). The open‐field test was undertaken on P29 and P45. On P48, the animals were weighed, and the plantaris muscle was collected and its weight and length were measured. Transverse sections were stained with haematoxylin‐eosin, NADH‐TR, Masson's trichrome and non‐specific esterase reaction for analysis. and transmission electron microscopy was performed. In the CP group, reductions were observed in mobility time, number of crossings and rearing frequency, body weight, muscle weight and length, and nucleus‐to‐fibre and capillary‐to‐fibre ratios. There was a statistically significant increase in the percentage area of the muscle section occupied by collagen; reduction in the area and increase in the number of type I muscle fibres; increase in myofibrillar disorganization and Z‐line disorganization and dissolution; and reduction in the area and largest and smallest diameters of neuromuscular junctions. Thus this animal model of CP produced morphofunctional alterations in skeletal muscle, that were associated with evidence of motor deficits as demonstrated by the open‐field test.
Databáze:	OpenAIRE
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