Hfq regulates anti-oxidative ability in Vibrio parahaemolyticus
| Autor: | Nagakatsu Harada, Yumi Harada, Akira Takahashi, Akiko Hamamoto, Zehong Su, Takaaki Shimohata, Tetsuro Koga, Xin Lian, Kazuaki Mawatari, Masayuki Nakano, Yutaka Nakaya, Masatake Akutagawa |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
chemistry.chemical_classification
biology Strain (chemistry) Superoxide Dismutase Vibrio parahaemolyticus Host Factor 1 Protein Catalase biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Superoxide dismutase Oxidative Stress Enzyme chemistry Gene expression biology.protein medicine Gene Oxidative stress |
| Zdroj: | The Journal of General and Applied Microbiology. 56:181-186 |
| ISSN: | 1349-8037 0022-1260 |
| DOI: | 10.2323/jgam.56.181 |
| Popis: | Hfq plays a fundamental role in bacterial cell physiology. It can stimulate or repress the expression of certain target genes, and there is a possibility that Hfq regulates the oxidative stress response. However, how Hfq functions that in Vibrio parahaemolyticus remains speculative. In this paper, we explain the functions Hfq plays in V. parahaemolyticus in the gene expression of superoxide dismutase gene and catalase gene, comparing the hfq deletion mutant strain to the parental strain. The results show that the hfq deletion mutant V. parahaemolyticus has a stronger ability to resist H(2)O(2). Superoxide dismutase (SOD) and catalase (CAT) activities in the hfq deletion mutant were remarkably higher than in the parental strain. Genetic experiments indicated that the gene expression of sod and kat was up-regulated in the mutant strain. These results indicate that Hfq down-regulates CAT and SOD activity, and Hfq is associated with the oxidative stress response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|