Supplementary FIGURE 1 from Repo-Man/protein phosphatase 1 SUMOylation mediates binding to lamin A and serine 22 dephosphorylation

Autor: Huguet, Florentin, Gokham, Ezgi, Foster, Helen A., Amin, Hasnat A., Vagnarelli, Paola
Rok vydání: 2022
Předmět:
DOI: 10.6084/m9.figshare.19410304.v1
Popis: A) Representative images of HeLa cells stained for lamin A S22ph (red) and DAPI. A metaphase (top panel), an ana/telophase (middle panel) and a cytokinesis (bottom panel) are shown. Scale bar 10 ��m. B) Representative images of the experiment quantified in Figure 3 E. HeLa cells were treated with RepoMan siRNA oligos, transfected with either the oligo-resistant versions of GFP:RepoManwt or GFP:RepoManIRCE mutant (green) for 36h then fixed and stained for anti lamin A S22ph (red). C) Representative of the experiment quantified in Figure 3 G. PLA conducted in HeLa cells transfected with either GFP:RepoManwt or GFP:RepoManIRCE mutant. The primary antibodies were anti GFP and anti laminAS22.
Databáze: OpenAIRE