Material surface engineering for multiplex cell culture in microwell
| Autor: | Valentina Spampinato, Christophe A. Marquette, Giacomo Ceccone, Céline A. Mandon, Guillaume G. Octobre, Benjamin P. Corgier, Ophélie I. Berthuy, Loïc J. Blum |
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| Přispěvatelé: | Génie Enzymatique, Membrane Biomimétique et Assemblages Supramoléculaires (GEMBAS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), AXO Science (Axo S), Depierre, Frédérique |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Materials science
Population Surface engineering CALCIUM chemistry.chemical_compound General Materials Science ALGINATES Polycarbonate Thin film GOLD films [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] education MICROENCAPSULATION [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] SPIN coating chemistry.chemical_classification Spin coating education.field_of_study Mechanical Engineering CELL culture Polymer Combinatorial chemistry chemistry Chemical engineering Mechanics of Materials Cell culture visual_art visual_art.visual_art_medium POLYSTYRENE Polystyrene MICROSTRUCTURE |
| Zdroj: | Journal of Materials Science Journal of Materials Science, Springer Verlag, 2014, 49 (13), pp.4481 |
| ISSN: | 0022-2461 1573-4803 |
| Popis: | International audience; In this study, we develop a new concept for multiplexed and localized cell co-culture. This cell chip consists of a polystyrene spin-coated solid support bearing gold-bottomed microwells. The cell-chip support is fabricated as follows: (i) electrosputtering of a thin layer of gold (40 nm) onto a polycarbonate substrate, (ii) spin coating of a polystyrene thin film (500 ± 50 nm) over the gold layer, followed by (iii) polystyrene etching through the spotting of toluene nanovolume (300-900 pL). In each gold-bottomed microwell, a small population of adherent cells (approx. 100 cells) can be cultured. In this miniaturized system, different cell lines can be co-cultured on a 1-cm surface, opening the way to multiplexed cell-chip development. In order to keep the cells in a properly hydrated environment and to physically retain them before they adhere, a biocompatible alginate polymer was used during the robotized micropipetting. This approach allows for the encapsulation of the cell in a very small volume (50 nL), directly in the microwells. After 24 h of culture, the cells adhered on the gold bottom of the microwells, and the alginate matrix was removed by addition of calcium-free culture medium. Graphical Abstract: Multiplex culture of cells was obtained using in situ produced microwells and encapsulated cells. The microwells are produced by organic solvent etching (nanovolume spotting) of a spin-coated polystyrene thin film, and the living multiple cell line deposition is obtained using on-site encapsulation in an alginate bead. [Figure not available: see fulltext.] |
| Databáze: | OpenAIRE |
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