Absence of the gut microbiota enhances anxiety-like behavior and neuroendocrine response to acute stress in rats

Autor: Michèle Crumeyrolle-Arias, Valérie Daugé, Sylvie Rabot, Mathilde Jaglin, Ana Cardona, Aurélia Bruneau, Laurent Naudon, Sylvie Vancassel
Přispěvatelé: Physiopathologie des Maladies du Système Nerveux Central, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Nutrition et Régulation Lipidique des Fonctions Cérébrales, Institut National de la Recherche Agronomique (INRA), Imagerie Dynamique (Plate-Forme) (PFID), Institut Pasteur [Paris] (IP), INRA, Nutrition, Chemical Food Safety and Consumer Behavior Division (ANSSD), French Ministry of Higher Education and Research, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Institut Pasteur [Paris]
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Male
Corticotropin-Releasing Hormone
Endocrinology
Diabetes and Metabolism
Dopamine
[SDV]Life Sciences [q-bio]
Hippocampus
Pituitary-Adrenal System
Hypothalamic pituitary adrenal axis
Glucocorticoid receptor
Anxiety
Open field
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Open-field
Corticosterone
Corticotropin releasing factor
0303 health sciences
Behavior
Animal
Brain monoamines
Microbiota
Gut–brain interaction
Dopaminergic
Brain
Intestines
Psychiatry and Mental health
medicine.anatomical_structure
Psychology
Hypothalamic–pituitary–adrenal axis
medicine.drug
medicine.medical_specialty
Hypothalamo-Hypophyseal System
digestive system
03 medical and health sciences
Receptors
Glucocorticoid
Internal medicine
medicine
Animals
Social Behavior
Germfree
Biological Psychiatry
030304 developmental biology
Endocrine and Autonomic Systems
Dentate gyrus
Rats
Inbred F344
Rats
Monoamine neurotransmitter
chemistry
030217 neurology & neurosurgery
Stress
Psychological
Zdroj: Psychoneuroendocrinology
Psychoneuroendocrinology, 2014, 42, pp.207-217. ⟨10.1016/j.psyneuen.2014.01.014⟩
Psychoneuroendocrinology, Elsevier, 2014, 42, pp.207-217. ⟨10.1016/j.psyneuen.2014.01.014⟩
ISSN: 0306-4530
Popis: Background and aims: Establishment of the gut microbiota is one of the most important events in early life and emerging evidence indicates that the gut microbiota influences several aspects of brain functioning, including reactivity to stress. To better understand how the gut microbiota contributes to a vulnerability to the stress-related psychiatric disorders, we investigated the relationship between the gut microbiota, anxiety-like behavior and HPA axis activity in stress-sensitive rodents. We also analyzed the monoamine neurotransmitters in the brain upper structures involved in the regulation of stress and anxiety. Methods: Germfree (GF) and specific pathogen free (SPF) F344 male rats were first subjected to neurological tests to rule out sensorimotor impairments as confounding factors. Then, we examined the behavior responses of rats to social interaction and open-field tests. Serum corticosterone concentrations, CRF mRNA expression levels in the hypothalamus, glucocorticoid receptor (GR) mRNA expression levels in the hippocampus, and monoamine concentrations in the frontal cortex, hippocampus and striatum were compared in rats that were either exposed to the open-field stress or not. Results: GF rats spent less time sniffing an unknown partner than SPF rats in the social interaction test, and displayed a lower number of visits to the aversive central area, and an increase in latency time, time spent in the corners and number of defecations in the open-field test. In response to the open-field stress, serum corticosterone concentrations were 2.8-fold higher in GF than in SPF rats. Compared to that of SPF rats, GF rats showed elevated CRF mRNA expression in the hypothalamus and reduced GR mRNA expression in the hippocampus. GF rats also had a lower dopaminergic turnover rate in the frontal cortex, hippocampus and striatum than SPF rats. Conclusions: In stress-sensitive F344 rats, absence of the gut microbiota exacerbates the neuroendocrine and behavioral responses to acute stress and the results coexist with alterations of the dopaminergic turnover rate in brain upper structures that are known to regulate reactivity to stress and anxiety-like behavior. (C) 2014 Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
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