Gentamicin-attenuated Leishmania infantum vaccine: protection of dogs against canine visceral leishmaniosis in endemic area of southeast of Iran
Autor: | Sarah Cleaveland, Hossein Kamiabi, Stephen Phillips, Mohammad Javad Namazi, Hamid Daneshvar, Richard Burchmore |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962 Serology parasitic diseases medicine biology business.industry lcsh:Public aspects of medicine Public Health Environmental and Occupational Health Vaccine trial Biology and Life Sciences Leishmaniasis lcsh:RA1-1270 medicine.disease biology.organism_classification Virology Sandfly Vaccination Infectious Diseases Immunology biology.protein Veterinary Science Gentamicin Antibody Leishmania infantum business Research Article medicine.drug |
Zdroj: | PLoS Neglected Tropical Diseases, Vol 8, Iss 4, p e2757 (2014) PLoS Neglected Tropical Diseases |
ISSN: | 1935-2735 1935-2727 |
Popis: | An attenuated line of Leishmania infantum (L. infantum H-line) has been established by culturing promastigotes in vitro under gentamicin pressure. A vaccine trial was conducted using 103 naive dogs from a leishmaniosis non-endemic area (55 vaccinated and 48 unvaccinated) brought into an endemic area of southeast Iran. No local and/or general indications of disease were observed in the vaccinated dogs immediately after vaccination. The efficacy of the vaccine was evaluated after 24 months (4 sandfly transmission seasons) by serological, parasitological analyses and clinical examination. In western blot analysis of antibodies to L. infantum antigens, sera from 10 out of 31 (32.2%) unvaccinated dogs, but none of the sera from vaccinated dogs which were seropositive at >100, recognized the 21 kDa antigen of L. infantum wild-type (WT). Nine out of 31 (29%) unvaccinated dogs, but none of vaccinated dogs, were positive for the presence of Leishmania DNA. One out of 46 (2.2%) vaccinated dogs and 9 out of 31 (29%) unvaccinated dogs developed clinical signs of disease. These results suggest that gentamicin-attenuated L. infantum induced a significant and strong protective effect against canine visceral leishmaniosis in the endemic area. Author Summary A 24 month vaccine trial was conducted using 103 leishmania free dogs in an area of southeast Iran endemic for visceral leishmaniosis. The dogs were vaccinated with gentamicin-attenuated line of Leishmania infantum. No local and/or general indications of disease were observed in the vaccinated dogs immediately after vaccination with an attenuated line of Leishmania infantum. Nine out of 31 (29%) unvaccinated dogs, but none of those vaccinated, were positive for presence of Leishmania DNA by PCR. In western blots, sera from 10 out of 31 (32.2%) unvaccinated dogs, but none of the sera from vaccinated dogs, recognized the 21 kDa antigen of Leishmania infantum wild-type. One out of 46 (2.2%) vaccinated dogs and 9 out of 31 (29%) unvaccinated dogs developed clinical signs of disease. The attenuated Leishmania infantum induced a significant and strong protective effect against Leishmania infantum infection in the field. |
Databáze: | OpenAIRE |
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