Identification and characterization of the Hfq bacterial amyloid region DNA interactions
| Autor: | Omar El Hamoui, Florent Busi, Christophe Sandt, Florian Turbant, David Partouche, Frank Wien, Véronique Arluison |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Messenger RNA Amyloid Chemistry Biophysics DNA:protein fibrils Translation (biology) QD415-436 QH426-470 Protein aggregation Bacterial adaptation Biochemistry Amino acid chemistry.chemical_compound Functional amyloid Genetics Nucleic acid Electrophoretic mobility shift assay DNA induced protein fibrillation Bacterial amyloid DNA Research Article |
| Zdroj: | BBA Adv BBA Advances, Vol 1, Iss, Pp 100029-(2021) |
| ISSN: | 2667-1603 |
| DOI: | 10.1016/j.bbadva.2021.100029 |
| Popis: | Nucleic acid amyloid proteins interactions have been observed in the past few years. These interactions often promote protein aggregation. Nevertheless, molecular basis and physiological consequences of these interactions are still poorly understood. Additionally, it is unknown whether the nucleic acid promotes the formation of self-assembly due to direct interactions or indirectly via sequences surrounding the amyloid region. Here we focus our attention on a bacterial amyloid, Hfq. This protein is a pleiotropic bacterial regulator that mediates many aspects of nucleic acids metabolism. The protein notably mediates mRNA stability and translation efficiency by using stress-related small non coding regulatory RNA. In addition, Hfq, thanks to its amyloid C-terminal region, binds and compacts DNA. A combination of experimental methodologies, including synchrotron radiation circular dichroism (SRCD), gel shift assay and infrared (FTIR) spectroscopy have been used to probe the interaction of Hfq C-terminal region with DNA. We clearly identify important amino acids in this region involved in DNA binding and polymerization properties. This allows to understand better how this bacterial amyloid interacts with DNA. Possible functional consequence to answer to stresses are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|