ROS-induced autophagy reduces B16F10 melanoma cell proliferative activity
| Autor: | Pedro J.L. Crugeira, Susana C. P. S. de Oliveira, Neandder A. Correia, Fernando José Pires Sampaio, Sandra R.C. de A. Fagnani, Gustavo Miranda Pires Santos, Juliana C. S. Monteiro, Antonio L. B. Pinheiro |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Programmed cell death medicine.medical_treatment Cell Melanoma Experimental Photodynamic therapy Apoptosis Dermatology Fluorescence 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Cell Line Tumor medicine Autophagy Animals Humans DAPI Cell Proliferation Photosensitizing Agents Chemistry Cell growth Melanoma Lasers Cell Membrane medicine.disease Methylene Blue 030104 developmental biology medicine.anatomical_structure Photochemotherapy 030220 oncology & carcinogenesis Cancer research Surgery Reactive Oxygen Species |
| Zdroj: | Lasers in medical science. 33(6) |
| ISSN: | 1435-604X |
| Popis: | Cancer is a pathology characterized by increased cell progression and/or reduced programmed cell death. Melanoma shows a rapid increase in cell progression and its resistance to chemotherapy is associated with uncontrolled apoptosis and to mechanisms that increase the flow of the drug out of the cell. The objective of this study was to evaluate the effects of photodynamic therapy (PDT) on the cell proliferation and cellular alterations in B16F10 murine melanoma. For that, four experimental groups were evaluated: the control group; laser group (ʎ = 660 ηm, 40 mW, 2.4 J/cm2); photosensitizer group (solution containing methylene blue and toluidine blue 1:1–12.5 μg/mL); PDT group. The incubation time was 30 min. Fluorescence microscopy assays were performed without fixation with the DAPI, monodansylcadaverine (MDC), and dihydroethidium (DHE) probes. Cell proliferation was also determined at 24-h time. The tests were performed in triplicate and the statistical test used was ANOVA with Tukey post-test. The results demonstrate that the plasma membrane of the cells of all the experimental groups remained intact, ROS production and autophagy significantly increased (p |
| Databáze: | OpenAIRE |
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