Intracellular protein degradation in cultures of dystrophic muscle cells and fibroblasts
| Autor: | Mary Ann McElligott, J. Fred Dice |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Male
animal structures Proteolysis medicine.medical_treatment Duchenne muscular dystrophy Muscle Proteins Human skin Chick Embryo Protein degradation Biology Muscular Dystrophies medicine Animals Humans Insulin Cells Cultured medicine.diagnostic_test Myogenesis Muscles Dystrophy Proteins Cell Biology Fibroblasts Muscular Dystrophy Animal medicine.disease Molecular biology Protein catabolism Kinetics Blood embryonic structures |
| Zdroj: | Experimental cell research. 150(2) |
| ISSN: | 0014-4827 |
| Popis: | We have analysed protein degradation in primary cultures of normal and dystrophic chick muscle, in fibroblasts derived from normal and dystrophic chicks, and in human skin fibroblasts from normal donors and from patients with Duchenne muscular dystrophy (DMD). Our results indicate that degradative rates of both short- and long-lived proteins are unaltered in dystrophic muscle cells and in dystrophic fibroblasts. Longer times in culture and co-culturing chick fibroblasts with the chick myotubes do not expose any dystrophy-related abnormalities in protein catabolism. Furthermore, normal and dystrophic muscle cells and fibroblasts are equally able to regulate proteolysis in response to serum and insulin. We conclude that cultures of chick myotubes, chick fibroblasts, and fibroblasts derived from humans afflicted with DMD are not appropriate models for studying the enhanced protein degradation observed in dystrophy. |
| Databáze: | OpenAIRE |
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