Anticholinesterase drugs stimulate smooth muscle contraction of the rat trachea through the Rho-kinase pathway
| Autor: | Tetsuji Makita, Koji Sumikawa, Kenji Nishioka, Osamu Shibata, Maki Yoshimura, Masakazu Yamaguchi, Masataka Saito |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Contraction (grammar) Pharmacology In Vitro Techniques Protein Serine-Threonine Kinases chemistry.chemical_compound Myosin medicine Animals Phosphatidylinositol Rats Wistar Rho-associated protein kinase Protein Kinase Inhibitors rho-Associated Kinases Dose-Response Relationship Drug business.industry Fasudil Intracellular Signaling Peptides and Proteins Muscle Smooth Smooth muscle contraction Neostigmine Rats Trachea Anesthesiology and Pain Medicine chemistry Anesthesia Cholinesterase Inhibitors medicine.symptom business medicine.drug Muscle contraction Muscle Contraction |
| Zdroj: | Anesthesia and analgesia. 102(4) |
| ISSN: | 1526-7598 |
| Popis: | We performed this study to determine the effects of Rho-kinase inhibitors, Y-27632 and fasudil, on the anticholinesterase (anti-ChE)-induced contractile and phosphatidylinositol responses of the rat trachea. In vitro measurements of isometric tension and [3H] inositol monophosphate (IP1) that was formed were conducted by using rat tracheal rings or slices. Neostigmine- and pyridostigmine-induced contractions were almost completely inhibited by Y-27632 and fasudil at 30 microM each, whereas acetylcholine-induced contraction was inhibited incompletely, i.e., by 56% by Y-27632 and by 51% by fasudil, at 100 microM for each, respectively. The inhibitory effects of fasudil on neostigmine- and acetylcholine-induced contractions were completely reversed by calyculin-A, a myosin phosphatase inhibitor. Neostigmine-induced IP1 accumulation was attenuated by fasudil at 100 microM. The results suggest that anti-ChEs cause airway smooth muscle contraction, in part, through activation of the Rho-kinase pathway. |
| Databáze: | OpenAIRE |
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