Heterogeneous expression of dopaminergic markers and Vglut2 in mouse mesodiencephalic dopaminergic nuclei A8-A13
| Autor: | Maxime Fougère, Joël Boutin, Dimitri Ryczko, Cornelis Immanuel van der Zouwen |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Biology Cell fate determination 03 medical and health sciences Glutamatergic Mice 0302 clinical medicine Dopamine Mesencephalon mental disorders medicine Animals Zebrafish Dopamine transporter Tyrosine hydroxylase General Neuroscience Dopaminergic Neurons Dopaminergic Glutamate receptor biology.organism_classification Cell biology Mice Inbred C57BL 030104 developmental biology biology.protein Vesicular Glutamate Transport Protein 2 Female 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | The Journal of comparative neurologyREFERENCES. 529(7) |
| ISSN: | 1096-9861 |
| Popis: | Co-transmission of glutamate by brain dopaminergic (DA) neurons was recently proposed as a potential factor influencing cell survival in models of Parkinson's disease. Intriguingly, brain DA nuclei are differentially affected in Parkinson's disease. Whether this is associated with different patterns of co-expression of the glutamatergic phenotype along the rostrocaudal brain axis is unknown in mammals. We hypothesized that, as in zebrafish, the glutamatergic phenotype is present preferentially in the caudal mesodiencephalic DA nuclei. Here, we used in mice a cell fate mapping strategy based on reporter protein expression (ZsGreen) consecutive to previous expression of the vesicular glutamate transporter 2 (Vglut2) gene, coupled with immunofluorescence experiments against tyrosine hydroxylase (TH) or dopamine transporter (DAT). We found three expression patterns in DA cells, organized along the rostrocaudal brain axis. The first pattern (TH-positive, DAT-positive, ZsGreen-positive) was found in A8-A10. The second pattern (TH-positive, DAT-negative, ZsGreen-positive) was found in A11. The third pattern (TH-positive, DAT-negative, ZsGreen-negative) was found in A12-A13. These patterns should help to refine the establishment of the homology of DA nuclei between vertebrate species. Our results also uncover that Vglut2 is expressed at some point during cell lifetime in DA nuclei known to degenerate in Parkinson's disease and largely absent from those that are preserved, suggesting that co-expression of the glutamatergic phenotype in DA cells influences their survival in Parkinson's disease. |
| Databáze: | OpenAIRE |
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