In vitro reconstruction of inflammatory reaction in human semen: effect on sperm DNA fragmentation
| Autor: | Maciej Kurpisz, Grzegorz Dworacki, Dorota Sanocka, Monika Fraczek, Anna Szumala-Kakol |
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| Rok vydání: | 2013 |
| Předmět: |
Male
DNA damage Bacteroidaceae Immunology Semen DNA Fragmentation Semen inflammation Biology Obstetrics and Gynaecology Bacteroidaceae Infections Escherichia coli Humans Immunology and Allergy Comet assay Cells Cultured Escherichia coli Infections Infertility Male Differential centrifugation TUNEL assay Obstetrics and Gynecology Molecular biology Sperm Reproductive Medicine Sperm Motility DNA fragmentation Cytokines Inflammation Mediators Percoll |
| Zdroj: | Journal of Reproductive Immunology. 100(1):76-85 |
| ISSN: | 0165-0378 |
| DOI: | 10.1016/j.jri.2013.09.005 |
| Popis: | The study was aimed at evaluating an in vitro induction of DNA damage in three sperm subpopulations exposed to selected inflammatory mediators, such as leukocytes, two combinations of pro-inflammatory cytokines (interleukin [IL]-6+IL-8 and IL-12+IL-18) and two bacterial strains (Escherichia coli and Bacteroides ureolyticus). Semen samples from normozoospermic volunteers were differentiated by swim-up (swim-up fraction) and Percoll gradient procedures (90% and 47% Percoll fractions). Leukocytes were isolated from the whole heparinized blood using the density gradient centrifugation technique. DNA fragmentation in sperm fractions was evaluated using flow cytometry with TUNEL labeling and Comet assay. Out of the inflammatory factors tested, bacteria were found to have a greatest toxic effect on sperm DNA, especially in fractions isolated by Percoll gradient, compared with untreated cells (P |
| Databáze: | OpenAIRE |
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