Evaluation of days-dependent chloramphenicol dosage on rat liver microsomal lipid peroxidation and catalase activity
| Autor: | A.A. Adesuyi, S.N.B. Ukoh, E.O. Farombi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Reactive oxygen species Antioxidant biology Chemistry Thiobarbituric acid Chloramphenicol medicine.medical_treatment Lipid peroxidation chemistry.chemical_compound medicine.anatomical_structure Endocrinology Catalase Internal medicine Hepatocyte biology.protein medicine TBARS Chloramphenicol rat liver lipid peroxidation catalase activity antioxidant medicine.drug |
| Zdroj: | Journal of Agriculture, Forestry and the Social Sciences; Vol 14, No 2 (2016); 80-86 |
| ISSN: | 1597-0906 |
| Popis: | Drugs and chemical agents can alter the cellular functions associated with the oxidative metabolism, thereby stimulating ROS production. The objective of the this study was the in vivo study of the inhibitory effect of chloramphenicol on hepatic microsomal enzyme system and the effect on lipid peroxidation using the thiobarbituric acid (TBA) reactive as index of peroxidation damage was investigated. The rats were randomly divided into 3 groups; group 1 serves as the control receiving saline water, group 2 receive chloramphenicol at a dose of 28.6 mg/kg body weight/day for 5 days and group 3 for 7 days. Liver protein content, lipid hydroperoxides and catalase activity were all determined. TBARS concentration increased statistically significantly with time of exposure. After 5 days of treatment, the level of TBARS increased by 103.56% with exogenous oxidant and by 141.54% for without oxidant, as compared to the respective control. On day 7, TBARS level in the liver was approximately 90.75% higher than in the control group with oxidant and 117.03% higher than the control without oxidant. The antibiotic elicited significant increase in rat liver lipid peroxidation compared to control. There were decreases in microsomal protein content of the liver in the drug-treated rats when compared with the control rats. It also showed that chloramphenicol treatment affects cytosolic catalase activities. It decreases catalase levels by 15.75% in 5 days dosage treatment and 17.81% in 7 days dosage treatment respectively. There were significance difference (P |
| Databáze: | OpenAIRE |
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