Investigation of the Utility of Complementary Electrochemical Detection Techniques to Examine the in Vitro Affinity of Bacterial Flagellins for a Toll-Like Receptor 5 Biosensor
| Autor: | Heinz-Bernhard Kraatz, Nora W. C. Chan, Kristin Topping, Nan Wang, Mohtashim H. Shamsi, Zhe She |
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| Rok vydání: | 2015 |
| Předmět: |
Salmonella typhimurium
Permittivity biology Chemistry Nanotechnology Biosensing Techniques Electrochemical Techniques Electrochemical detection Bacillus subtilis Electrochemistry biology.organism_classification In vitro Analytical Chemistry Dielectric spectroscopy Toll-Like Receptor 5 Scanning electrochemical microscopy Biosensor Flagellin |
| Zdroj: | Analytical Chemistry. 87:4218-4224 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | An initial investigation of the fabrication of a novel biosensor utilizing toll-like receptor 5 (TLR5) has been conducted. The detection assay using this sensor platform has been carried out using two complementary electrochemical techniques. The electrochemical properties of the modified bare gold surface following TLR5 immobilization were characterized. The electrochemical response to changes in the sensor film resistance and electron charge-transfer permittivity triggered by independent exposures to flagellins from Salmonella typhimurium (S. typhimurium) and Bacillus subtilis (B. subtilis) were examined and observed. The quantified film resistance data gathered using electrochemical impedance spectroscopy (EIS) over a macroscopic scale are in significant agreement with the corresponding electron charge-transfer permittivity measured locally by scanning electrochemical microscopy (SECM). Unlike other sensors that exploit pathogen recognition elements, TLR5 biosensors have the potential to carry out broad-spectrum detection of flagellated bacterial pathogens in near real time. This broad-spectrum detection platform is a significant step toward the development of fast, inexpensive clinical tools for early warning diagnoses and immediate on-site treatment. |
| Databáze: | OpenAIRE |
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