Inferred protein content and distribution from density measurements of calcitic and aragonitic otoconia
| Autor: | Robert H. Kretsinger, Christian Weber, Kenneth G. Pote |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Aragonite
Proteins Mineralogy Biology engineering.material Sensory Systems Calcium Carbonate Rats Protein content Otolithic Membrane Xenopus laevis chemistry.chemical_compound medicine.anatomical_structure Calcium carbonate chemistry Centrifugation Density Gradient Microscopy Electron Scanning engineering medicine Animals sense organs Biomineralization Otolith |
| Zdroj: | Hearing Research. 66:225-232 |
| ISSN: | 0378-5955 |
| DOI: | 10.1016/0378-5955(93)90142-n |
| Popis: | Otoconia from the peripheral portion of the vestibular system contain specific proteins and are mineralized by several polymorphs of calcium carbonate. To infer the internal distribution of their mineral and protein components, we have measured the densities, by equilibrium centrifugation, and the lengths of aragonitic otoconia from the African clawed frog (Xenopus laevis) and calcitic otoconia from the Norway rat (Rattus norvegicus). The densities are statistically dependent on the length of the otoconia. Further, this dependence is not the same for aragonitic and calcitic otoconia. Aragonitic otoconia are statistically more dense when smaller and less dense when larger. For calcitic otoconia the opposite is true; the smaller otoconia are statistically less dense than larger otoconia. Because the organic and inorganic phases have different densities, this indicates that the otoconial proteins and the mineral phases have different distributions within these two types of otoconia. |
| Databáze: | OpenAIRE |
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