Critical reevaluation of the 4-(dimethylamino)cinnamaldehyde assay: Cranberry proanthocyanidin standard is superior to procyanidin A2 dimer for accurate quantification of proanthocyanidins in cranberry products
| Autor: | Jessica Parker, Xin Chen, Christina Khoo, Jannie P.J. Marais, P.M. Crump, Nathan J. Chesmore, Christian G. Krueger, Jess D. Reed, Dhanansayan Shanmuganayagam |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
TheoryofComputation_COMPUTATIONBYABSTRACTDEVICES Dimer DMAC Medicine (miscellaneous) 01 natural sciences Cinnamaldehyde 03 medical and health sciences chemistry.chemical_compound MALDI-TOF MS Proanthocyanidins TX341-641 Cranberry (Vaccinium macrocarpon Ait) 030109 nutrition & dietetics Nutrition and Dietetics Chromatography Nutrition. Foods and food supply 010401 analytical chemistry TheoryofComputation_GENERAL Structural heterogeneity 0104 chemical sciences 4- (dimethylamino)cinnamaldehyde ComputingMethodologies_PATTERNRECOGNITION chemistry Proanthocyanidin Procyanidin A2 Food Science |
| Zdroj: | Journal of Functional Foods, Vol 22, Iss, Pp 13-19 (2016) |
| ISSN: | 1756-4646 |
| DOI: | 10.1016/j.jff.2016.01.017 |
| Popis: | The 4-(dimethylamino)cinnamaldehyde (DMAC) assay is currently used to quantify proanthocyanidin (PAC) content in cranberry products. In a multi-operator/multi-day study design, a cranberry proanthocyanidin (c-PAC) standard was compared to procyanidin A2 (ProA2) dimer for accurate quantification of PAC in commercial cranberry juices, lab generated cranberry blends and cranberry powders. The c-PAC standard reflects the structural heterogeneity of cranberry PAC degree of polymerization, hydroxylation pattern and ratios of ‘A-type’ to ‘B-type’ interflavanyl bonds. Use of the c-PAC standard to quantify PAC content in cranberry samples resulted in values that were 3.6 times higher than those determined by ProA2. Overall, there was no effect (P > 0.05) of operator or day on estimation of PAC concentration. The adoption of c-PAC standard should be considered as an improvement over the use of ProA2 for accurate quantification of cranberry PAC. Improved standardization of bioactive PAC components in functional cranberry foods will aid in establishment of dosage guidelines. |
| Databáze: | OpenAIRE |
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